Given the information provided, please answer the following question: Volume of cell suspension needed (for 5 x 104 cells): __________________microliters (µL)
Given the information provided, please answer the following question: Volume of cell suspension needed (for 5 x 104 cells): __________________microliters (µL)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Given the information provided, please answer the following question: Volume of cell suspension needed (for 5 x 104 cells): __________________microliters (µL)
![Determine the number of cells you have per ml of your cell suspension.
Number of cells counted in quadrant 1 =
46
Number of cells counted in quadrant 2 =
38
Number of cells counted in quadrant 3 =
56
Number of cells counted in quadrant 4 =
38
TOTAL number of cells for ALL 4 quadrants =
178
Number of cells per ml will equal:
[(total # of cells counted) divided by 4] X 104 =,
scientific notation)
_445,000_
cells/ml (use
B. Setting up a new culture of cells
1. You will need to determine the volume of your cell suspension needed to obtain 5 x 10^4 cells.
Use the value for cells per ml you determined above in Part A.
Example: If your cells per ml = 2 x 105
5 x 104 cells
= 0.25 ml, or 250 ul suspension
2 x 105 cells/ml
5 x 10^4 cells
0.12235955
= Volume of cell suspension needed:
mL
cells/mL
Volume of cell suspension needed (for 5 x 104 cells):
_microliters (uL)
These cells will be used to prepare "protein lysate" for western blot.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F6929eb17-4ffa-4323-bed9-d33e82c7ec91%2F05a6a7db-0d78-4816-9ce3-74a200aca191%2F98igprr_processed.png&w=3840&q=75)
Transcribed Image Text:Determine the number of cells you have per ml of your cell suspension.
Number of cells counted in quadrant 1 =
46
Number of cells counted in quadrant 2 =
38
Number of cells counted in quadrant 3 =
56
Number of cells counted in quadrant 4 =
38
TOTAL number of cells for ALL 4 quadrants =
178
Number of cells per ml will equal:
[(total # of cells counted) divided by 4] X 104 =,
scientific notation)
_445,000_
cells/ml (use
B. Setting up a new culture of cells
1. You will need to determine the volume of your cell suspension needed to obtain 5 x 10^4 cells.
Use the value for cells per ml you determined above in Part A.
Example: If your cells per ml = 2 x 105
5 x 104 cells
= 0.25 ml, or 250 ul suspension
2 x 105 cells/ml
5 x 10^4 cells
0.12235955
= Volume of cell suspension needed:
mL
cells/mL
Volume of cell suspension needed (for 5 x 104 cells):
_microliters (uL)
These cells will be used to prepare "protein lysate" for western blot.
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