Given the information above and the absorption data below determine the reaction rate for this reaction. Time (s) Abs. at 340nm Time (s) Abs. at 340nm 0.991 40 0.501 0.926 45 0.439 10 0.863 50 0.378 15 0.798 55 0.318 20 0.738 60 0.258 25 0.684 65 0.197 30 0.623 70 0.135 35 0.562
Given the information above and the absorption data below determine the reaction rate for this reaction. Time (s) Abs. at 340nm Time (s) Abs. at 340nm 0.991 40 0.501 0.926 45 0.439 10 0.863 50 0.378 15 0.798 55 0.318 20 0.738 60 0.258 25 0.684 65 0.197 30 0.623 70 0.135 35 0.562
Principles of Modern Chemistry
8th Edition
ISBN:9781305079113
Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Publisher:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Chapter18: Chemical Kinetics
Section: Chapter Questions
Problem 65AP
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Question
Please show the calculation
![Enzymes often react with zero-order reaction kinetics. The activity of glutamate-oxaloacetate transaminase
(GOT) can be measured by coupling the production of oxaloacetate to BOT to the oxidation of NADH using
malate dehydrogenase (MDH). By monitoring the absorption value at 340 nm at 37°C we can determine the
reaction rate for this reaction.
For a zero-order reaction the following relationship can be used to determine the reaction rate:
At
= kt + Ao
Where
• Ag is the absorption at time =t
• Ao is the absorption at time = 0
k is the reaction rate](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F8eb4bbb1-ce19-4609-90d7-2fd844e3c584%2Fee796f3c-9a6d-4169-8bd2-f9d72f9864c7%2Fzuoxv2w_processed.png&w=3840&q=75)
Transcribed Image Text:Enzymes often react with zero-order reaction kinetics. The activity of glutamate-oxaloacetate transaminase
(GOT) can be measured by coupling the production of oxaloacetate to BOT to the oxidation of NADH using
malate dehydrogenase (MDH). By monitoring the absorption value at 340 nm at 37°C we can determine the
reaction rate for this reaction.
For a zero-order reaction the following relationship can be used to determine the reaction rate:
At
= kt + Ao
Where
• Ag is the absorption at time =t
• Ao is the absorption at time = 0
k is the reaction rate
![Given the information above and the absorption data below determine the reaction rate for this reaction.
Time (s)
Abs. at 340nm
Time (s)
Abs. at 340nm
0.991
40
0.501
0.926
45
0.439
10
0.863
50
0.378
15
0.798
55
0.318
20
0.738
60
0.258
25
0.684
65
0.197
30
0.623
70
0.135
35
0.562](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F8eb4bbb1-ce19-4609-90d7-2fd844e3c584%2Fee796f3c-9a6d-4169-8bd2-f9d72f9864c7%2Fmu099k_processed.png&w=3840&q=75)
Transcribed Image Text:Given the information above and the absorption data below determine the reaction rate for this reaction.
Time (s)
Abs. at 340nm
Time (s)
Abs. at 340nm
0.991
40
0.501
0.926
45
0.439
10
0.863
50
0.378
15
0.798
55
0.318
20
0.738
60
0.258
25
0.684
65
0.197
30
0.623
70
0.135
35
0.562
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