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For the four tubes used in today's experiment (lab handout, page 23), which tube is used as negative control? O Tube #1 O Tube #2 Tube #3 Tube #4

For the four tubes used in today's experiment (lab handout, page 23), which tube is used as negative control? O Tube #1 O Tube #2 Tube #3 Tube #4

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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## The Reaction Procedure ### Materials Needed: - Four microtubes (0.5ml or 1.5ml) - Waterproof marking pen - Microliter dispenser - Laboratory slides ### Instructions: 1. **Labeling Microtubes:** Obtain four microtubes (0.5ml or 1.5ml) and label them #1 - #4 with a waterproof marking pen. 2. **Adding Solutions:** Using a microliter dispenser, add the following quantities to the tubes: | Tube # | Con A-buffer | Con A-Peroxidase | Galactose | Mannose | |--------|--------------|------------------|-----------|---------| | 1 | 35 μl | 0 | 0 | 0 | | 2 | 10 μl | 25 μl | 0 | 0 | | 3 | 0 | 25 μl | 10 μl | 0 | | 4 | 0 | 25 μl | 0 | 10 μl | 3. **Mixing Solutions:** Mix the solutions by tapping the tubes with the tip of your index finger. 4. **Dispensing Solutions:** Place the slides on the laboratory bench, top surface up, and dispense 15 μl of the solution in tube #1 onto slide #1, 15 μl of the solution in tube #2 onto slide #2, 15 μl of the solution in tube #3 onto slide #3, and 15 μl of the solution in tube #4 onto slide #4. ### Diagram Explanation (No diagram present): Based on the text instructions, there is no specific diagram provided. The written instructions should be sufficient to guide the user through the process. In summary, the protocol involves preparing four different microtube solutions with specific reagent volumes, mixing these solutions properly, and then dispensing them onto corresponding slides for further analysis or reaction observations.
Transcribed Image Text:## The Reaction Procedure ### Materials Needed: - Four microtubes (0.5ml or 1.5ml) - Waterproof marking pen - Microliter dispenser - Laboratory slides ### Instructions: 1. **Labeling Microtubes:** Obtain four microtubes (0.5ml or 1.5ml) and label them #1 - #4 with a waterproof marking pen. 2. **Adding Solutions:** Using a microliter dispenser, add the following quantities to the tubes: | Tube # | Con A-buffer | Con A-Peroxidase | Galactose | Mannose | |--------|--------------|------------------|-----------|---------| | 1 | 35 μl | 0 | 0 | 0 | | 2 | 10 μl | 25 μl | 0 | 0 | | 3 | 0 | 25 μl | 10 μl | 0 | | 4 | 0 | 25 μl | 0 | 10 μl | 3. **Mixing Solutions:** Mix the solutions by tapping the tubes with the tip of your index finger. 4. **Dispensing Solutions:** Place the slides on the laboratory bench, top surface up, and dispense 15 μl of the solution in tube #1 onto slide #1, 15 μl of the solution in tube #2 onto slide #2, 15 μl of the solution in tube #3 onto slide #3, and 15 μl of the solution in tube #4 onto slide #4. ### Diagram Explanation (No diagram present): Based on the text instructions, there is no specific diagram provided. The written instructions should be sufficient to guide the user through the process. In summary, the protocol involves preparing four different microtube solutions with specific reagent volumes, mixing these solutions properly, and then dispensing them onto corresponding slides for further analysis or reaction observations.
### Negative Control Identification - Educational Activity **Question:** For the four tubes used in today's experiment (lab handout, page 23), which tube is used as the negative control? **Options:** - ◯ Tube #1 - ◯ Tube #2 - ◯ Tube #3 - ◯ Tube #4 **Instructions:** Select the appropriate tube that is designated as the negative control for the experiment. Refer to the lab handout on page 23 if needed to make your selection. The negative control is crucial as it serves as a baseline to compare the results of the experimental tubes and ensures the experiment's validity by showing no reaction when no reaction is expected.
Transcribed Image Text:### Negative Control Identification - Educational Activity **Question:** For the four tubes used in today's experiment (lab handout, page 23), which tube is used as the negative control? **Options:** - ◯ Tube #1 - ◯ Tube #2 - ◯ Tube #3 - ◯ Tube #4 **Instructions:** Select the appropriate tube that is designated as the negative control for the experiment. Refer to the lab handout on page 23 if needed to make your selection. The negative control is crucial as it serves as a baseline to compare the results of the experimental tubes and ensures the experiment's validity by showing no reaction when no reaction is expected.
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