Which of the following method isemployed in the sample preparation forthe cryo-electron microscope?O The sample is flash-frozen in water to formcrystalline iceO The sample is embedded, sectioned andthen stained with a negative stain thenflash-frozen in water to form vitreous oramorphous iceThe sample is flash-frozen in water to formvitreous or amorphous iceThe sample is embedded, sectioned andthen stained with a negative stain thenflash-frozen in water to form crystalline ice

Answered: Which of the following method is…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Which of the following method is
employed in the sample preparation for
the cryo-electron microscope?
O The sample is flash-frozen in water to form
crystalline ice
O The sample is embedded, sectioned and
then stained with a negative stain then
flash-frozen in water to form vitreous or
amorphous ice
The sample is flash-frozen in water to form
vitreous or amorphous ice
The sample is embedded, sectioned and
then stained with a negative stain then
flash-frozen in water to form crystalline ice

Expert Solution

Step 1: Introduction

Cryo-electron microscopy (Cryo-EM) is an advanced imaging technique used to visualize the structures of biological molecules and specimens at high resolution. It involves rapidly freezing samples in a way that preserves them in a non-crystalline, vitreous ice state. This method allows scientists to examine the intricate details of biological molecules and complexes, such as proteins, viruses, and cells, with exceptional clarity and without the need for staining or sectioning, providing valuable insights into their structures and functions.

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