For GC analysis, the following can cause band broadening and poorresolutionUse of automatic samplerSlow injection and oversize samplesUse of 5 m or longer packed columnFor samples of fatty acid methyl esters, alkaloids, drugs, andhalogenated compounds, an applicable liquid stationary phase for GLCisPolyethylene glycol50% Cyanopropyl-polydimethyl siloxane5% Phenyl-polydimethyl siloxaneA C18 column is an example of a "reverse phase" column. Reverse phasecolumns are often used with more polar solvents such as water,methanol or acetonitrile. The stationary phase is a nonpolarhydrocarbon, whereas the mobile phase is a polar liquid. If a sample hasthe following components, what will be the order of elution? (A.) 1,6-Hexadiol (B.) 2,5-Hexadiol (C.) 2,3,4-HexatriolCBABCAABC

Chromatography is separating technique for mixtures into it's components. It is based on the…

For GC analysis, the following can cause band broadening and poor resolution OUse of automatic sampler Slow injection and oversize samples O Use of 5 m or longer packed column For samples of fatty acid methyl esters, alkaloids, drugs, and halogenated compounds, an applicable liquid stationary phase for GLC is Polyethylene glycol 50% Cyanopropyl-polydimethyl siloxane 5% Phenyl-polydimethyl siloxane

For GC analysis, the following can cause band broadening and poor resolution OUse of automatic sampler Slow injection and oversize samples O Use of 5 m or longer packed column For samples of fatty acid methyl esters, alkaloids, drugs, and halogenated compounds, an applicable liquid stationary phase for GLC is Polyethylene glycol 50% Cyanopropyl-polydimethyl siloxane 5% Phenyl-polydimethyl siloxane

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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The following sentence has the format “Statement 1 BECAUSE Statement 2”. Decide whether statement 1 is true, whether statement 2 is true, and whether the REASONING (the ‘BECAUSE’ relationship) between them is valid. Statement 1 − “Cyclohexane-1,2-diol elutes from a silica gel chromatography column after cyclohexane-1,2-dione” BECAUSE Statement 2 − “Ketones stick better to silica than alcohols because the C=O group has a larger dipole than the C-OH group.” Question 6 options: A Statement 1 and Statement 2 are both correct, and the reasoning is valid. B Statement 1 and Statement 2 are both correct, but the reasoning is NOT valid. C Statement 1 is correct, but Statement 2 is NOT correct. D Statement 1 is NOT correct, but Statement 2 is correct. E Statement 1 and Statement 2 are both NOT correct
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DATA Calculate Rf values for each component Solvent Iron Paper Chromatography Copper Nickel Group 1 100 mm 90 mm 70 mm 30 mm Group 2 100 mm 88mm 68 mm 32 mm STANDARDS Iron 90mm Copper 72mm Nickel 32 Solvent 100 mm Group 3 100 mm 90 mm 72 mm 34 mm
Table 2. Optimum temperature determination for novel a-amylase Volume of 0.1% Volume of pH 6.7 buffer (mL) Test Volume of 0.9% Pre-incubation Absorbance Tube w/v starch (mL) NacI (mL) (°C) 1 3.50 1.00 0.50 30 0.276 2 3.50 1.00 0.50 40 0.320 3 3.50 1.00 0.50 50 0.422 4 3.50 1.00 0.50 60 0.288 3.50 1.00 0.50 70 0.124 6. 3.50 1.00 0.50 80 0.195 B. One drop (negligible volume) of a relatively concentrated enzyme solution was added in each test tube. After ten minutes, the reactions were terminated and one drop of DNS reagent (negligible volume) was added. Absorbance were measured at the same wavelength above. What is the optimum temperature for the amylase enzyme? Justify your answer.|