Table 2. Optimum temperature determination for novel a-amylase Volume of pH 6.7 buffer (mL) Test Volume of 0.1% Volume of 0.9% Pre-incubation Absorbance Tube w/v starch (mL) NacI (mL) (°C) 1 3.50 1.00 0.50 30 0.276 3.50 1.00 0.50 40 0.320 3 3.50 1.00 0.50 50 0.422 4 3.50 1.00 0.50 60 0.288 3.50 1.00 0.50 70 0.124 3.50 1.00 0.50 80 0.195 B. One drop (negligible volume) of a relatively concentrated enzyme solution was added in each test tube. After ten minutes, the reactions were terminated and one drop of DNS reagent (negligible volume) was added. Absorbance were measured at the same wavelength above. What is the optimum temperature for the amylase enzyme? Justify your answer. LO

Table 2. Optimum temperature determination for novel a-amylase Volume of pH 6.7 buffer (mL) Test Volume of 0.1% Volume of 0.9% Pre-incubation Absorbance Tube w/v starch (mL) NacI (mL) (°C) 1 3.50 1.00 0.50 30 0.276 3.50 1.00 0.50 40 0.320 3 3.50 1.00 0.50 50 0.422 4 3.50 1.00 0.50 60 0.288 3.50 1.00 0.50 70 0.124 3.50 1.00 0.50 80 0.195 B. One drop (negligible volume) of a relatively concentrated enzyme solution was added in each test tube. After ten minutes, the reactions were terminated and one drop of DNS reagent (negligible volume) was added. Absorbance were measured at the same wavelength above. What is the optimum temperature for the amylase enzyme? Justify your answer. LO

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Similar questions

#1?
▪ True or false: the internal standard behaves chemically just like the analyte, and it is not found in the sample(it is added) ▪ True or false: In analysis of blood alcohols, lead is a much better IS than n-propanol
3
Calculate the pH of two HCl solution based on their concentrations of 0.001 and0.0001 M. Then calculate the pH of a solution when you add each of these to a closed carbonicacid system with mcarb-t = mH2CO3 + mHCO3- + mCO32- = 0.01 m and pH = 6. At that pH, mH2CO3 =10-2.16; mHCO3- =10-2.51; mCO32- is negligible.
Compare and contrast the amount of caffeine obtained from the single vs multipleextractions with Red Bull and Coca-Cola beverages. Explain why this has occured. Single Extractions: TEST SOLUTION NAME ABSORBANCE READING [CAFFEINE]mg/100ml 5ml Red Bull 2.412 57.9mg/100ml 5ml Coca-Cola 0.716 16.2mg/100ml Multiple Extractions: TEST SOLUTION NAME ABSORBANCE READING [CAFFEINE]mg/100ml 10ml Red Bull 3.253 78.453mg/100ml 10ml Coca-Cola 0.785 17.844mg/100ml
29. What exactly is relative retention and retention time? How do you solve problems like this?
Sample # 1 2 0.2425 0.2160 0.2274 Weighed mass of ascorbic acid (g) volume of KIO3 (mL) 18.86 17.91 17.37 0.02459 0.02462 0.02432 [KIO3] (mol L-1) Mean [KIO3] (based on titration) 0.02451 mol L-1 Standard deviation in the mean [KIO3] mol L-1 Confidence limit for the [KI03] mol L-1 Relative confidence limit for the [KIO3] ppt =
Total volume = 100mL
For an analysis of phthalates by GC-MS, the limits of detection and quantitation are 0.55 and 3.56 mg/g, respectively. Based on the results obtained below, how are the 3 samples reported? Sample Concentration (mg/g) A 4.50 B 0.055 C 0.56
Draw Lewis structure(s) for the acetate ion (CH2CO0). If there are equivalent resonance structures, draw all of them. • Draw one structure per sketcher box, and separate added sketcher boxes with the Do not include overall ion charges or formal charges in your drawing. • Do not draw double bonds to oxygen unless they are needed in order for the central atom to obey the octet rule. symbol. CH3COO":
Please answer this question.
1) Calculate the total hardness for the first sample (Sample Volume,V : 50 mL,Volume of EDTA titrant used for the sample, A:9.6 mL) 2)Calculate the calcium and magnesium concentrations for the first sample in the video in meq/L and mg CaCO3/L.(Sample Volume= 50 mL, Magnesium hardness titrant volume = 0 mL, Calcium hardness titrant volume:2,6 mL) 3)Assuming the pH and alkalinity of the first sample in the video are 7.5 and 100 mg CaCO3/L, respectively, calculate the temporary and permanent hardness values for this sample 4How much CaCL2 in meq/L should be added to the sample to equalize calcium and magnesium hardness? Which hardness level (soft, medium, hard, very hard) does the sample belong to after this addition?