For each of the restriction enzymes listed below:(i) Approximately how many restriction fragmentswould result from digestion of the human genome(3 × 109bases) with the enzyme? (ii) Estimate theaverage size of the pieces of the human genomeproduced by digestion with the enzyme. (iii) Statewhether the fragments of human DNA produced bydigestion with the given restriction enzyme wouldhave sticky ends with a 5′ overhang, sticky endswith a 3′ overhang, or blunt ends. (iv) If the enzymeproduces sticky ends, would all the overhangs on allthe ends produced on all fragments of the humangenome with that enzyme be identical, or not? (Therecognition sequence on one strand for each enzymeis given in parentheses, with the 5′ end written atthe left. N means any of the four nucleotides; R is any purine—that is, A or G; and Y is any pyrimidine—that is, C or T. ^ marks the site of cleavage.)a. Sau3A (^GATC)b. BamHI (G^GATCC)c. HpaII (C^CGG)d. SphI (GCATG^C)e. NaeI (GCC^GGC)f. BanI (G^GYRCC)g. BstYI (R^GATCY)h. BslI (CCNNNNN^NNGG)i. SbfI (CCTGCA^GG)
For each of the restriction enzymes listed below:
(i) Approximately how many restriction fragments
would result from digestion of the human genome
(3 × 109
bases) with the enzyme? (ii) Estimate the
average size of the pieces of the human genome
produced by digestion with the enzyme. (iii) State
whether the fragments of human DNA produced by
digestion with the given restriction enzyme would
have sticky ends with a 5′ overhang, sticky ends
with a 3′ overhang, or blunt ends. (iv) If the enzyme
produces sticky ends, would all the overhangs on all
the ends produced on all fragments of the human
genome with that enzyme be identical, or not? (The
recognition sequence on one strand for each enzyme
is given in parentheses, with the 5′ end written at
the left. N means any of the four
a. Sau3A (^GATC)
b. BamHI (G^GATCC)
c. HpaII (C^CGG)
d. SphI (GCATG^C)
e. NaeI (GCC^GGC)
f. BanI (G^GYRCC)
g. BstYI (R^GATCY)
h. BslI (CCNNNNN^NNGG)
i. SbfI (CCTGCA^GG)
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