For a particular bacterial strain, the molecular formula was determined to be C4.4H7.301.2No.86- These bacterial cells are grown under aerobic conditions with hexadecane (C16H34) as substrate. Assume that 60% of the hexadecane is used for producing cells (also called biomass) and the remaining 40% of the hexadecane is used for other cell functions. You have been put in charge of a small batch fermenter for growing the bacteria and aim to produce 10 kg of cells for inoculation of a pilot-scale reactor. a) Assuming this 60% conversion, what is the yield of cells from hexadecane in kg/kg? b) What minimum amount of hexadecane substrate (in kg) must be contained in your culture medium? Remember that only 60% of the hexadecane is converted to cells. c) What must be the minimum concentration of hexadecane (in kg/m³) in the medium if the fermenter working volume is 4.3 cubic meters? Remember that only 60% of the hexadecane is converted to cells.

For a particular bacterial strain, the molecular formula was determined to be C4.4H7.301.2No.86- These bacterial cells are grown under aerobic conditions with hexadecane (C16H34) as substrate. Assume that 60% of the hexadecane is used for producing cells (also called biomass) and the remaining 40% of the hexadecane is used for other cell functions. You have been put in charge of a small batch fermenter for growing the bacteria and aim to produce 10 kg of cells for inoculation of a pilot-scale reactor. a) Assuming this 60% conversion, what is the yield of cells from hexadecane in kg/kg? b) What minimum amount of hexadecane substrate (in kg) must be contained in your culture medium? Remember that only 60% of the hexadecane is converted to cells. c) What must be the minimum concentration of hexadecane (in kg/m³) in the medium if the fermenter working volume is 4.3 cubic meters? Remember that only 60% of the hexadecane is converted to cells.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Concept explainers

Mass Spectrometry

This process is an instrumental method in which a sample is changed to fast moving positive ions by electron bombardment. The charged particles vary due to their different mass to charge ratio and are estimated with the help of this difference. It is also called mass spectroscopy.

Question

2. Custom problem: Stoichiometry, yield and extent of reaction
We have fermenter where the following biological reaction is taking place:
C16H34 + 16.28 O₂ +1.42 NH3 →1.65 C4.4H7.301.2No.86 +8.74 CO₂ + 13.11 H₂O
For a particular bacterial strain, the molecular formula was determined to be C4.4H7.301.2N0.86-
These bacterial cells are grown under aerobic conditions with hexadecane (C16H34) as substrate.
Assume that 60% of the hexadecane is used for producing cells (also called biomass) and the
remaining 40% of the hexadecane is used for other cell functions. You have been put in charge of a
small batch fermenter for growing the bacteria and aim to produce 10 kg of cells for inoculation of
a pilot-scale reactor.
a) Assuming this 60% conversion, what is the yield of cells from hexadecane in kg/kg?
b) What minimum amount of hexadecane substrate (in kg) must be contained in your culture
medium? Remember that only 60% of the hexadecane is converted to cells.
c)
What must be the minimum concentration of hexadecane (in kg/m³) in the medium if the
fermenter working volume is 4.3 cubic meters? Remember that only 60% of the hexadecane
is converted to cells.

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