Fluorescent FM dyes partition reversibly into biological membranes without penetrating through them. Suppose that you have neurons cultured in a dish, and you incubate them with an FM dye for a period of time before washing the cells with excess medium to remove the dye. a) You find that if you stimulate the neurons to trigger action potentials during incubation with the dye, the synaptic regions of the neurons remain fluorescent after the wash. Propose an explanation for this effect. b) After you have generated fluorescent synaptic regions by this procedure, suppose that you trigger further action potentials while continuing to wash with excess medium. Would you expect the synaptic regions to lose fluorescence? Explain.
Fluorescent FM dyes partition reversibly into biological membranes without penetrating through them. Suppose that you have neurons cultured in a dish, and you incubate them with an FM dye for a period of time before washing the cells with excess medium to remove the dye. a) You find that if you stimulate the neurons to trigger action potentials during incubation with the dye, the synaptic regions of the neurons remain fluorescent after the wash. Propose an explanation for this effect. b) After you have generated fluorescent synaptic regions by this procedure, suppose that you trigger further action potentials while continuing to wash with excess medium. Would you expect the synaptic regions to lose fluorescence? Explain.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Fluorescent FM dyes partition reversibly into biological membranes without penetrating through them. Suppose that you have neurons cultured in a dish, and you incubate them with an FM dye for a period of time before washing the cells with excess medium to remove the dye. a) You find that if you stimulate the neurons to trigger action potentials during incubation with the dye, the synaptic regions of the neurons remain fluorescent after the wash. Propose an explanation for this effect. b) After you have generated fluorescent synaptic regions by this procedure, suppose that you trigger further action potentials while continuing to wash with excess medium. Would you expect the synaptic regions to lose fluorescence? Explain.
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