Firefly luciferase is the enzyme that allows fireflies to illuminate their abdomens. Because this light generation is an ATP-requiring reaction, firefly luciferase can be used to test for the presence of ATP. In this way, luciferase can test for the presence of life. The coupled reactions are 1. luciferin + O2 = oxyluciferin + light 2. ATP = AMP + PP; AG°=-47.99 kJ/mol If the overall AG° of the coupled reaction is -5 kJ/mol , what is the standard free energy of the first reaction at 27°C? Express your answer numerically to 1 decimal place.

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Firefly luciferase is the enzyme that allows fireflies to illuminate their
abdomens. Because this light generation is an ATP-requiring reaction, firefly
luciferase can be used to test for the presence of ATP. In this way, luciferase
can test for the presence of life. The coupled reactions are
1. luciferin + O2 = oxyluciferin + light
2. ATP = AMP + PP; AG°=-47.99 kJ/mol
If the overall AG' of the coupled reaction is -5 kJ/mol , what is the standard free
energy of the first reaction at 27°C?
Express your answer numerically to 1 decimal place.
Transcribed Image Text:Firefly luciferase is the enzyme that allows fireflies to illuminate their abdomens. Because this light generation is an ATP-requiring reaction, firefly luciferase can be used to test for the presence of ATP. In this way, luciferase can test for the presence of life. The coupled reactions are 1. luciferin + O2 = oxyluciferin + light 2. ATP = AMP + PP; AG°=-47.99 kJ/mol If the overall AG' of the coupled reaction is -5 kJ/mol , what is the standard free energy of the first reaction at 27°C? Express your answer numerically to 1 decimal place.
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