Fill in the blank 1. A genetically engineered rodent in which a normal gene has been replaced by a a defective version of the same gene is called ______. 2. DNA fingerprints (ex: in forensics) can be generated by selectively cutting DNA with bacterially derived proteins called _______ and examining the resulting DNA fragment patterns on agarose gels.
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- Activity 2. Think Like a Scientist Answer the following scenario like you are the experts in the field of genetic engineering. Write your answers below. A molecular geneticist hopes to find a gene in human liver cells that codes for an important blood- clotting protein. He knows that the nucleotide sequence of a small part of the gene is GTGGACTGACA. Briefly explain how to obtain the desired gene.ansenh. Will save this response. Quèstion 13 Which shows the correct complementary base pairing for DNA? O 1.C-A, T-G O 2.C-G, U-A O 3. A-G, C-T O 4. T-A, G-C O 5. T-A, G-U A Moving to another question will save this response. ARGenetically Modified Foods The creation of transgenic crop plants using recombinant DNA methods involves the transfer of just one gene or a small number of genes to the plants, in contrast to classical breeding methods in which hundreds or even thousands of genes are transferred at once. Explain why this is true. If fewer genes are transferred during the creation of transgenic crops, why are some people afraid that they are dangerous?
- Match. This is a diagram showing..? The structure identified by A The structure identified by B 1. plasmid 2. normal bacterial cell division 3. mRNA 4. horizontal gene transfer 5. Bacterial DNA in a bacteriophage 6. a virus such as SARS-CoV-2Need help, please. Please answer the following four questions to the image I attached. 1. You digest both plasmids with BamHI and SacI together (a double digest). What size fragments will result? Please indicate the sizes of the expected fragments in basepairs: The large fragment of pNUT is fragment A. This fragment is expected to be ____ bp. 2. The small fragment of pNUT is fragment B. This fragment is expected to be ____ bp. 3. The large fragment of pPOD is fragment C. This fragment is expected to be ____ bp. 4. The small fragment of pPOD is fragment D. This fragment is expected to be ____ bp.8 points total. Within the general field of biotechnology, DNA technology uses modern laboratory techniques for the studying and manipulation of genetic material. Explain how DNA might be sequenced, analyzed, and "cut and pasted" as DNA technology is employed. In addition, outline one way in which DNA technology could be employed to improve human lives. I 3
- Write TRUE or FALSE. If false, write the word/s that make(s) the statement incorrect. 1.The primosome complex includes primase, helicase and gyrase. 2.In plasmid DNA biotechnology, plasmid inserted with a “correct" DNA sequence is transferred to bacteria for rapid.Fill the blanks. 1. In Polymerase Chain Reaction (PCR), after one round of PCR, one molecule of DNA consisting of two complementary strands yields (BLANK) molecules of DNA for a total of (BLANK) strands. 2i. How many DNA molecules would exist after 2 PCR cycles? (BLANK) ii. 5 cycles? (BLANK) iii. 10 cycles? (BLANK) iv. 20 cycles? (BLANK) v. 30 cycles? (BLANK)LO 64- Explain how Next gen sequencing is applied in different technologies In which of the following scenario would it be best to use Next-gen sequencing? (select all that apply) a-To separate fragments of DNA based on their size- b-To insert a mutation at random in a gene c-To idently a novel pathogen d-To determine the nucleotide sequence of a microbe e- To insert methyl groups to cytosines in a DNA sequence asap please
- genetics question 3. Explain how accurate direct-to-consumer test kits are for identifying BRCA1 and BRCA2 mutations? If you had a positive result, how would you interpret this result and what steps might you take next?Please answer all correctly. Thanks. 1. When DNA helicase is active, the result is: - annealing of RNA primers to the DNA - formation of phosphodiester bonds - formation of hydrogen bonds - separation of the two strands of the double helix - swivelling of the single separated strands of DNA 2. During DNA replication _______ aids in relieving pressure from DNA supercoiling. - helicase - RNA polymerase - ligase - topoisomeraseMultiple Matching. Match the term with its description.___________nucleic acid probe___________nontemplate strand___________template strand___________reverse transcriptase___________Taq polymerase ___________RNAi___________primer___________restriction endonucleasea. enzyme that transcribes RNA into DNAb. complementary strand that blocks mRNA expressionc. the nontranslated strand of DNA or RNAd. enzyme that snips DNA at palindromese. oligonucleotide that initiates the PCRf. strand of nucleic acid that is transcribed or translatedg. thermostable enzyme for synthesizing DNAh. oligonucleotide used in hybridization
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