Fig. 5: LAPTM5 transports HIV-1 Env to the lysosome for degradation. a 120, x43 x 137 x146 NL4-3 Vpr- 100- 80- 60- 8 40- 20- Bal Vpr- d Mock gp120 150- Mock 400, x70 x 98 х66 50- 300- T gp41 200- 120, 100- * 80- 60- 40- 50- Pr55-Gag 37- 100- LAPTM5 37- Mock LAPTM5 0.5 GAPDH 1.0 C 0.5 1.0 Vector (ug) 20- Bal Vpr- No Treat. BFLA Treat LAPTMS CLQ LAPTM5 NL4-3 Bal AD8 LAPTM5 Bal Vpr- 150- Mock Vpr- gp120 50- Lysosome lysates Cell lysates Pr55-Gag 37- No Treat. BFLA LAPTM5 e 37- - \No Treat. BFLA GAPDH LAPTM5 Mock LAPTM5-GFP Mock Mock Env Gag DAPI 150- Merge gp120 50- Pr55-Gag LAPTM5 LAMP1 GFP 37- Env GAPDH Gag Bal Vpr- DAPI Merge gp120 (%) p24 (%) Mock LAPTM5 Mock LAPTM5 Mock Mock LAPTM5 No Treat. BFLA LAPTM5 LAPTM5 Mock LAPTM5 Mock LAPTM5 BFLA_ No Treat. * gp120 (ng/mL) BFLA LAPTM5 CLQ LAPTM5 LAPTM5
Fig. 5: LAPTM5 transports HIV-1 Env to the lysosome for degradation. a 120, x43 x 137 x146 NL4-3 Vpr- 100- 80- 60- 8 40- 20- Bal Vpr- d Mock gp120 150- Mock 400, x70 x 98 х66 50- 300- T gp41 200- 120, 100- * 80- 60- 40- 50- Pr55-Gag 37- 100- LAPTM5 37- Mock LAPTM5 0.5 GAPDH 1.0 C 0.5 1.0 Vector (ug) 20- Bal Vpr- No Treat. BFLA Treat LAPTMS CLQ LAPTM5 NL4-3 Bal AD8 LAPTM5 Bal Vpr- 150- Mock Vpr- gp120 50- Lysosome lysates Cell lysates Pr55-Gag 37- No Treat. BFLA LAPTM5 e 37- - \No Treat. BFLA GAPDH LAPTM5 Mock LAPTM5-GFP Mock Mock Env Gag DAPI 150- Merge gp120 50- Pr55-Gag LAPTM5 LAMP1 GFP 37- Env GAPDH Gag Bal Vpr- DAPI Merge gp120 (%) p24 (%) Mock LAPTM5 Mock LAPTM5 Mock Mock LAPTM5 No Treat. BFLA LAPTM5 LAPTM5 Mock LAPTM5 Mock LAPTM5 BFLA_ No Treat. * gp120 (ng/mL) BFLA LAPTM5 CLQ LAPTM5 LAPTM5
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Please help explain this figure
Transcribed Image Text:Fig. 5: LAPTM5 transports HIV-1 Env to the
lysosome for degradation.
a
b
120,
100-
* 80-
x43 x 137 x 146
NL4-3 Vpr-
Bal Vpr-
60-
응 40어
Mock
Mock
Mock
Mock
150-
4007 x70
20-
50-
gp120
E 300-T
x98
x 66
120,
100-
* 80-
60-
40-
20-
gp41
50-
200-
37-
Pr55-Gag
100-
37-
LAPTM5
0.5
1.0
GAPDH
0.5
Mock
1.0 Vector (pg)
Bal Vpr-
LAPTM5
No Treat. BFLA CLQ
LAPTM5
Mock
LAPTM5
Mock
LAPTM5
Mock
NL4-3 Bal AD8
Bal Vpr-
150-
Mock
Mock
Vpr-
gp120
50-
Lysosome
lysates
Cell lysates
Pr55-Gag
37-
BFLA
No Treat.
BFLA
LAPTM5
e
37-
LAPTM5-GFP
GAPDH
LAPTM5
150-
Env
Mock
Моcк
Gag
DAPI
LAPTM5
Merge
Mock
gp120
50-
37-
Pr55-Gag
LAPTM5
GFP
LAMP1
37-
Env
Gag
GAPDH
DAPI
Bal Vpr-
Merge
p24 (%)
LAPTM5
Мock
LAPTM5
LAPTM5
LAPTM5
No Treat.
LAPTM5
BFLA
LAPTM5
BFLA No Treat. *
LAPTM5
gp120 (ng/mL)
No Treat. LAPTM5
Мock
BFLA LAPTM5
CLQ
\No Treat.
LAPTM5
Mock
LAPTM5
Expert Solution
Step 1
"ANSWER
- By causing damage to HIV-1 Env, LAPTM5 transports it to the lysosome. With Vpr-defective HIV-1 proviral vectors, FLAG-tagged FLAG -5 or funny expressions were transformed into HeLa cells. p24 or gp120 ELISA were used to assess HIV-1 capsids or Env gp120-containing virion viruses two days after transmission.
- The SEM intermediate of three independent experiments is used to illustrate the data.
- HeLa cells are connected with LAPTM5 in the presence or absence of lysosomal inhibitors in the presence or absence of FLAG-tagged or fake compounds, as well as Vpr-defective HIV-1 proviral vectors (b); or (c) in the absence or presence of Vpr-defective HIV-1 proviral vectors (BFLA at 50 nM or CLQ in treatment 30 m in the last 24 hours).
- To evaluate the expression of LAPTM5, Gag, gp120, gp41, and GAPDH.
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