**Experiment 3: Effect of pH at a Constant Substrate Concentration and Temperature****Objective:**To investigate the effect of pH on the reaction rate while maintaining a constant substrate concentration and temperature.**Procedure, Experimental Result, and Data:**The experiment was conducted using five test tubes, each containing the same substrate concentration but subjected to different pH levels. The substrate concentration and temperature were kept constant throughout the experiment to isolate the effect of pH on the reaction rate.**Experimental Setup:**- Test Tube Configuration: - Each test tube contains 8.0 grams of substrate. - The contents are maintained at a constant temperature of 37°C. - Different pH levels are used for each test tube as follows: 1. Test Tube 1: pH 3 2. Test Tube 2: pH 5 3. Test Tube 3: pH 7 4. Test Tube 4: pH 9 5. Test Tube 5: pH 11**Visual Explanation:**- **Left Diagram:** - Shows the setup of five test tubes with varying amounts of substrate. - Test tube numbers correspond to substrate amounts: 0.5 g, 1.0 g, 2.0 g, 4.0 g, and 8.0 g.- **Center Diagram:** - Shows five test tubes labeled with different pH values (3, 5, 7, 9, 11) and their contents after reaction. - Indicates the color change due to the reaction.- **Right Table:** - Provides the detailed data of the experiment.**Data Table:**| Test Tube Number | Amount of Substrate (g) | Number of Molecules of Product Formed per Minute (×10^9) ||------------------|--------------------------|--------------------------------------------------------|| 1 | 8.0 | 96 || 2 | 8.0 | 198 || 3 | 8.0 | 350 || 4 | 8.0 | 223 || 5 | 8.0 | 121 |**Conclusion:**From the experimental results, it is evident that pH 7 yields the highest number of molecules of **Experiment 2: Effect of Substrate Concentration at a Constant pH (pH = 5)****Instructions:**Plot the data given in the procedure section using the **graph you made for Experiment 1** and answer the following questions.1. **What is the effect of decreasing pH on the rate of reaction?**2. **What is(are) the possible reason(s) that the number of molecules of product formed at pH 5 is less than that at pH 7?****Explanation of Graphs and Diagrams (if applicable):**Since there is no accompanying graph or diagram provided with the text, please refer to the graph made in **Experiment 1**, which should display the relationship between substrate concentration and reaction rate at varying pH levels. The graph will help visualize how changing the pH from 7 to 5 affects the number of molecules of product formed.For detailed analysis:- Ensure the x-axis of your graph plots substrate concentration.- Ensure the y-axis of your graph plots the rate of the reaction or the number of molecules of the product formed.- Compare the plot for pH 7 and pH 5 to answer the questions effectively.**Discussion Section:**When answering the questions, consider how enzyme activity and substrate affinity might change with pH, influencing the reaction rate and product formation. Evaluate the potential denaturation of the enzyme or alterations in the active site under different pH conditions.These questions are aimed at enhancing your understanding of the biochemical principles governing enzyme kinetics and the impact of environmental factors such as pH.

A question based on kinetics of reactions in solution that is to be accomplished.

Experiment 2: Effect of substrate concentration at a constant pH (pH = 5) Plot the data given in the procedure section using the graph you made for Experiment 1 and answer the following questions 1. What is the effect of decreasing pH on the rate of reaction? 2. What is (are) the possible reason(s) that the number of molecules of product formed at pH 5 less than that of pH 7?.

Experiment 2: Effect of substrate concentration at a constant pH (pH = 5) Plot the data given in the procedure section using the graph you made for Experiment 1 and answer the following questions 1. What is the effect of decreasing pH on the rate of reaction? 2. What is (are) the possible reason(s) that the number of molecules of product formed at pH 5 less than that of pH 7?.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Similar questions

Please don't provide handwriting solution
Do not give handwriting solution.
The compound shown below is the product of a Claisen condensation. Draw structural formulas for the reactants: ester and enolate ion. off • Assume all esters to be ethyl esters. Do not include counter-ions, e.g., Na*, I, in your answer. • Draw the enolate ion in its carbanion form. Draw one structure per sketcher. Add additional sketchers using the drop-down menu in the bottom right corner. • Separate multiple reactants using the + sign from the drop-down menu. ✓
In this experiment, we used DCM, Benzoic Acid and NaOH In what form is the organic acid after it is extracted? Draw the acid-base reaction using structures and arrows. Answer the same question for the organic base that was used and draw the reaction scheme for it as well. Upload a picture of your drawing (these should include mechanism arrows showing the movement of electrons).
Please help me with this question, it's urgent The reaction shown below does not follow the expected theory. Given what you learned about substitution/elimination, what is the expected mechanism this reaction should follow? Suggest why that is not what happens and give the actual product of the reaction.
CHỈ CH3C—NH2 a. Draw the structure of the tetrahedral intermediate INITIALLY-FORMED in the reaction shown. NaOH, H₂O • You do not have to consider stereochemistry. • Do not include counter-ions, e.g., Na+, I¯, in your answer. • In cases where there is more than one answer, just draw one. *8 MILL ? [F
Draw a detailed mechanism for the attached reaction done in the Griganrd Reaction experiment?
Question 2 ?
label all 4 identified regions in the active site, (labels like basic catalytic residue, acidic catalytic residue, catalytic nucleophile, etc.)
Write structures for the carbonyl electrophile and enolate nucleophile that react to give the aldol below. OH سعيد Draw the carbanion resonance structure for the enolate without a cation. Draw one structure per sketcher. Add additional sketchers using the drop-down menu in the bottom right corner. Separate structures with + signs from the drop-down menu.