**Experiment 3: Effect of pH at a Constant Substrate Concentration and Temperature****Objective:**To investigate the effect of pH on the reaction rate while maintaining a constant substrate concentration and temperature.**Procedure, Experimental Result, and Data:**The experiment was conducted using five test tubes, each containing the same substrate concentration but subjected to different pH levels. The substrate concentration and temperature were kept constant throughout the experiment to isolate the effect of pH on the reaction rate.**Experimental Setup:**- Test Tube Configuration: - Each test tube contains 8.0 grams of substrate. - The contents are maintained at a constant temperature of 37°C. - Different pH levels are used for each test tube as follows: 1. Test Tube 1: pH 3 2. Test Tube 2: pH 5 3. Test Tube 3: pH 7 4. Test Tube 4: pH 9 5. Test Tube 5: pH 11**Visual Explanation:**- **Left Diagram:** - Shows the setup of five test tubes with varying amounts of substrate. - Test tube numbers correspond to substrate amounts: 0.5 g, 1.0 g, 2.0 g, 4.0 g, and 8.0 g.- **Center Diagram:** - Shows five test tubes labeled with different pH values (3, 5, 7, 9, 11) and their contents after reaction. - Indicates the color change due to the reaction.- **Right Table:** - Provides the detailed data of the experiment.**Data Table:**| Test Tube Number | Amount of Substrate (g) | Number of Molecules of Product Formed per Minute (×10^9) ||------------------|--------------------------|--------------------------------------------------------|| 1 | 8.0 | 96 || 2 | 8.0 | 198 || 3 | 8.0 | 350 || 4 | 8.0 | 223 || 5 | 8.0 | 121 |**Conclusion:**From the experimental results, it is evident that pH 7 yields the highest number of molecules of **Experiment 2: Effect of Substrate Concentration at a Constant pH (pH = 5)****Instructions:**Plot the data given in the procedure section using the **graph you made for Experiment 1** and answer the following questions.1. **What is the effect of decreasing pH on the rate of reaction?**2. **What is(are) the possible reason(s) that the number of molecules of product formed at pH 5 is less than that at pH 7?****Explanation of Graphs and Diagrams (if applicable):**Since there is no accompanying graph or diagram provided with the text, please refer to the graph made in **Experiment 1**, which should display the relationship between substrate concentration and reaction rate at varying pH levels. The graph will help visualize how changing the pH from 7 to 5 affects the number of molecules of product formed.For detailed analysis:- Ensure the x-axis of your graph plots substrate concentration.- Ensure the y-axis of your graph plots the rate of the reaction or the number of molecules of the product formed.- Compare the plot for pH 7 and pH 5 to answer the questions effectively.**Discussion Section:**When answering the questions, consider how enzyme activity and substrate affinity might change with pH, influencing the reaction rate and product formation. Evaluate the potential denaturation of the enzyme or alterations in the active site under different pH conditions.These questions are aimed at enhancing your understanding of the biochemical principles governing enzyme kinetics and the impact of environmental factors such as pH.

A question based on kinetics of reactions in solution that is to be accomplished.

Experiment 2: Effect of substrate concentration at a constant pH (pH = 5) Plot the data given in the procedure section using the graph you made for Experiment 1 and answer the following questions 1. What is the effect of decreasing pH on the rate of reaction? 2. What is (are) the possible reason(s) that the number of molecules of product formed at pH 5 less than that of pH 7?.

Experiment 2: Effect of substrate concentration at a constant pH (pH = 5) Plot the data given in the procedure section using the graph you made for Experiment 1 and answer the following questions 1. What is the effect of decreasing pH on the rate of reaction? 2. What is (are) the possible reason(s) that the number of molecules of product formed at pH 5 less than that of pH 7?.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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