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EXPERIMENT 13: DETERMINATION OF DYE CONCENTRATION BY SPECTROPHOTOMETRY 1. You have been assigned solution number 3 to prepare. Calculate the concentration of Allura Red in this solution. 2. A student has found the following absorbance vs. concentration data. Concentration, M 9.80 x 10-6 6.35 x 10-6 4.12 x 10-6 1.43 x 10-6 Absorbance, A 0.810 0.522 0.333 0.101 Plot the absorbance (y-axis) versus concentration (x-axis) on graph paper. Draw the best straight line through the plotted points using a straight edge. Alternatively, plot the absorbance vs. concentration using a program such as Excel and do a least-squares fit to determine the best-fit line. Attach your graph to this pre-lab. 3. What is the slope of the best-fit line if taken from Excel? If done by hand, show your work. Remember to put the proper units on the slope.

EXPERIMENT 13: DETERMINATION OF DYE CONCENTRATION BY SPECTROPHOTOMETRY 1. You have been assigned solution number 3 to prepare. Calculate the concentration of Allura Red in this solution. 2. A student has found the following absorbance vs. concentration data. Concentration, M 9.80 x 10-6 6.35 x 10-6 4.12 x 10-6 1.43 x 10-6 Absorbance, A 0.810 0.522 0.333 0.101 Plot the absorbance (y-axis) versus concentration (x-axis) on graph paper. Draw the best straight line through the plotted points using a straight edge. Alternatively, plot the absorbance vs. concentration using a program such as Excel and do a least-squares fit to determine the best-fit line. Attach your graph to this pre-lab. 3. What is the slope of the best-fit line if taken from Excel? If done by hand, show your work. Remember to put the proper units on the slope.

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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EXPERIMENT 13: DETERMINATION OF DYE CONCENTRATION BY SPECTROPHOTOMETRY 1. You have been assigned solution number 3 to prepare. Calculate the concentration of Allura Red in this solution. 2. A student has found the following absorbance vs. concentration data. Concentration, M 9.80 x 10-6 6.35 x 10-6 4.12 x 10-6 1.43 x 10-6 Absorbance, A 0.810 0.522 0.333 0.101 Plot the absorbance (y-axis) versus concentration (x-axis) on graph paper. Draw the best straight line through the plotted points using a straight edge. Alternatively, plot the absorbance vs. concentration using a program such as Excel and do a least-squares fit to determine the best-fit line. Attach your graph to this pre-lab. 3. What is the slope of the best-fit line if taken from Excel? If done by hand, show your work. Remember to put the proper units on the slope. 4. The same student found an absorbance of 0.415 for an unknown solution. Find the concentration of the unknown solution in two ways. First, read the value off the graph. Show how you read the unknown value on the graph using dashed lines. Second, calculate the value using your slope and Beer's law. Show your work.
Transcribed Image Text:EXPERIMENT 13: DETERMINATION OF DYE CONCENTRATION BY SPECTROPHOTOMETRY 1. You have been assigned solution number 3 to prepare. Calculate the concentration of Allura Red in this solution. 2. A student has found the following absorbance vs. concentration data. Concentration, M 9.80 x 10-6 6.35 x 10-6 4.12 x 10-6 1.43 x 10-6 Absorbance, A 0.810 0.522 0.333 0.101 Plot the absorbance (y-axis) versus concentration (x-axis) on graph paper. Draw the best straight line through the plotted points using a straight edge. Alternatively, plot the absorbance vs. concentration using a program such as Excel and do a least-squares fit to determine the best-fit line. Attach your graph to this pre-lab. 3. What is the slope of the best-fit line if taken from Excel? If done by hand, show your work. Remember to put the proper units on the slope. 4. The same student found an absorbance of 0.415 for an unknown solution. Find the concentration of the unknown solution in two ways. First, read the value off the graph. Show how you read the unknown value on the graph using dashed lines. Second, calculate the value using your slope and Beer's law. Show your work.
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