Homework Help is Here – Start Your Trial Now!
Science
Chemistry
Exercise 1: Standard Curve for Proteln Measurements: A standard curve for protein concentration is often created using known concentrations of bovine serum albumin (protein). This process is called the Bradford Assay; it is a colorimetric assay. A special reagent turns blue when it binds to amino acids present in protein. The intensity of the color is best measured with a spectrophotometer (a device for comparing two light radiations, wavelength by wavelength). In the case of the Bradford Assay the greater the absorbance, the higher the protein concentration. A series of tests were performed on some samples and the following measurements were obtained using a spectrophotometer: Protein Concentration (mg/ml) 0.26 0.56 Absorbance (A) 0.098 0.213 0.84 0.383 1.12 0.473 0.527 1.40 TASKS: 1. Enter the data into Excel - the protein concentration is the independent variable. 2. Create a scatter graph of the data that is appropriately titled and labeled (see Introduction). 3. Use the Trendline feature of Excel to find the best linear fit to the data. 4. Display the equation and the R value of the trendline on the graph and print out the graph. 5. Print the graph, scaling so that it fits on an 8% x 11 in, sheet. 6. Use the equation for the standard curve to determine the protein concentrations of the following unknown samples: Absorbance (A)

Exercise 1: Standard Curve for Proteln Measurements: A standard curve for protein concentration is often created using known concentrations of bovine serum albumin (protein). This process is called the Bradford Assay; it is a colorimetric assay. A special reagent turns blue when it binds to amino acids present in protein. The intensity of the color is best measured with a spectrophotometer (a device for comparing two light radiations, wavelength by wavelength). In the case of the Bradford Assay the greater the absorbance, the higher the protein concentration. A series of tests were performed on some samples and the following measurements were obtained using a spectrophotometer: Protein Concentration (mg/ml) 0.26 0.56 Absorbance (A) 0.098 0.213 0.84 0.383 1.12 0.473 0.527 1.40 TASKS: 1. Enter the data into Excel - the protein concentration is the independent variable. 2. Create a scatter graph of the data that is appropriately titled and labeled (see Introduction). 3. Use the Trendline feature of Excel to find the best linear fit to the data. 4. Display the equation and the R value of the trendline on the graph and print out the graph. 5. Print the graph, scaling so that it fits on an 8% x 11 in, sheet. 6. Use the equation for the standard curve to determine the protein concentrations of the following unknown samples: Absorbance (A)

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
See similar textbooks
icon
Related questions
Question
100%
**Exercise 1: Standard Curve for Protein Measurements** A standard curve for protein concentration is often created using known concentrations of bovine serum albumin (protein). This process is called the Bradford Assay; it is a colorimetric assay. A special reagent turns blue when it binds to amino acids present in protein. The intensity of the color is best measured with a spectrophotometer (a device for comparing two light radiations, wavelength by wavelength). In the case of the Bradford Assay, the greater the absorbance, the higher the protein concentration. A series of tests were performed on some samples, and the following measurements were obtained using a spectrophotometer: | Protein Concentration (mg/ml) | Absorbance (A) | |------------------------------|----------------| | 0.26 | 0.098 | | 0.56 | 0.213 | | 0.84 | 0.383 | | 1.08 | 0.473 | | 1.40 | 0.527 | **TASKS:** 1. Enter the data into Excel—the protein concentration is the independent variable. 2. Create a scatter graph of the data that is appropriately titled and labeled (see Introduction). 3. Use the Trendline feature of Excel to find the best linear fit to the data. 4. Display the equation and the R² value of the trendline on the graph and print out the graph. 5. Print the graph, scaling so that it fits on an 8½ x 11 in. sheet. 6. Use the equation for the standard curve to determine the protein concentrations of the following unknown samples: - Absorbance (A) (Note: The table provided lists protein concentration in mg/ml alongside corresponding absorbance values, which helps create a standard curve to later determine unknown protein concentrations by measuring their absorbance.)
Transcribed Image Text:**Exercise 1: Standard Curve for Protein Measurements** A standard curve for protein concentration is often created using known concentrations of bovine serum albumin (protein). This process is called the Bradford Assay; it is a colorimetric assay. A special reagent turns blue when it binds to amino acids present in protein. The intensity of the color is best measured with a spectrophotometer (a device for comparing two light radiations, wavelength by wavelength). In the case of the Bradford Assay, the greater the absorbance, the higher the protein concentration. A series of tests were performed on some samples, and the following measurements were obtained using a spectrophotometer: | Protein Concentration (mg/ml) | Absorbance (A) | |------------------------------|----------------| | 0.26 | 0.098 | | 0.56 | 0.213 | | 0.84 | 0.383 | | 1.08 | 0.473 | | 1.40 | 0.527 | **TASKS:** 1. Enter the data into Excel—the protein concentration is the independent variable. 2. Create a scatter graph of the data that is appropriately titled and labeled (see Introduction). 3. Use the Trendline feature of Excel to find the best linear fit to the data. 4. Display the equation and the R² value of the trendline on the graph and print out the graph. 5. Print the graph, scaling so that it fits on an 8½ x 11 in. sheet. 6. Use the equation for the standard curve to determine the protein concentrations of the following unknown samples: - Absorbance (A) (Note: The table provided lists protein concentration in mg/ml alongside corresponding absorbance values, which helps create a standard curve to later determine unknown protein concentrations by measuring their absorbance.)
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps with 1 images

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
Recommended textbooks for you
Chemistry
Chemistry
Chemistry
ISBN:
9781305957404
Author:
Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry
Chemistry
Chemistry
ISBN:
9781259911156
Author:
Raymond Chang Dr., Jason Overby Professor
Publisher:
McGraw-Hill Education
Principles of Instrumental Analysis
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Organic Chemistry
Organic Chemistry
Chemistry
ISBN:
9780078021558
Author:
Janice Gorzynski Smith Dr.
Publisher:
McGraw-Hill Education
Chemistry: Principles and Reactions
Chemistry: Principles and Reactions
Chemistry
ISBN:
9781305079373
Author:
William L. Masterton, Cecile N. Hurley
Publisher:
Cengage Learning
Elementary Principles of Chemical Processes, Bind…
Elementary Principles of Chemical Processes, Bind…
Chemistry
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY
SEE MORE TEXTBOOKS