Draw a standard curve (using linear graph paper) of A595 (Y axis) versus μg protein for tubes 1-6 (standards) and use this to determine the amount of protein in each of the fractions (serum, B and crude IgG). Calculate the total amount of protein in the starting serum and the amount recovered in each of fraction B and the IgG fraction (you need to know the total volumes of these samples). The volume recorded for fraction c was 1ml and fraction b was 11ml. Please I need help
Draw a standard curve (using linear graph paper) of A595 (Y axis) versus μg protein for tubes 1-6 (standards) and use this to determine the amount of protein in each of the fractions (serum, B and crude IgG). Calculate the total amount of protein in the starting serum and the amount recovered in each of fraction B and the IgG fraction (you need to know the total volumes of these samples). The volume recorded for fraction c was 1ml and fraction b was 11ml. Please I need help
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
Draw a standard curve (using linear graph paper) of A595 (Y axis) versus μg protein for tubes 1-6 (standards) and use this to determine the amount of protein in each of the fractions (serum, B and crude IgG). Calculate the total amount of protein in the starting serum and the amount recovered in each of fraction B and the IgG fraction (you need to know the total volumes of these samples). The volume recorded for fraction c was 1ml and fraction b was 11ml. Please I need help
![Additions
Sample number
Standard (ul)
Water (ul)
Diluted Serum (µl)
Diluted B (ul)
IgG (Fraction C) (μl)
Fraction "D" (µl)
A595
Amount of protein
(ug) in assay tube
[protein] (ug/ul in the
diluted sample
[protein] (ug/ul)
undiluted sample
vol of undiluted
sample (ml)
Total protein in
undiluted sample (ug)
1
0
800
0
Protein standard
(bovine serum albumin, 1 mg/ml)
2
3
24
8
798 796 792
0.693 0.830 0.930
16
784
1.282
6
24
776
Diluted
Serum
795 790
5
1.465 0.740
10
Diluted
Fraction B
9
795 790
5 10
0.858 0.712
0.825
Diluted IgG
(Fraction C)
12
795 790
5 10
0.679
0.950
Diluted
fraction D
14
795 790
5 10
0.792
1.510](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fc6d3a15a-f17b-4374-9a9e-d74c30c6bef1%2F3fec228d-5d62-4e0b-8f23-ac178e794fb5%2Fxlcipeq_processed.png&w=3840&q=75)
Transcribed Image Text:Additions
Sample number
Standard (ul)
Water (ul)
Diluted Serum (µl)
Diluted B (ul)
IgG (Fraction C) (μl)
Fraction "D" (µl)
A595
Amount of protein
(ug) in assay tube
[protein] (ug/ul in the
diluted sample
[protein] (ug/ul)
undiluted sample
vol of undiluted
sample (ml)
Total protein in
undiluted sample (ug)
1
0
800
0
Protein standard
(bovine serum albumin, 1 mg/ml)
2
3
24
8
798 796 792
0.693 0.830 0.930
16
784
1.282
6
24
776
Diluted
Serum
795 790
5
1.465 0.740
10
Diluted
Fraction B
9
795 790
5 10
0.858 0.712
0.825
Diluted IgG
(Fraction C)
12
795 790
5 10
0.679
0.950
Diluted
fraction D
14
795 790
5 10
0.792
1.510
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 4 steps with 4 images
Recommended textbooks for you
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON