Done Gel Electrophoresis for the detect... Gel -Stacking ger -Resolving gel Glass plate Buffer Electrophoresis Cathode Anode Figure 4: Summary of Gel electrophoresis Please watch the following youtube videos on SDS-PAGE. https://www.youtube.com/watch?v=i_6y6Z5UvwE https://www.youtube.com/watch?v=On_ZotdZexI Please answer the following questions in the space given below: 1. What are the two types of gels used in poly acrylamide gel electrophoresis? 2. What is the role of SDS in SDS-PAGE? 3. Why is the gel soaked in coomassie stain?
Done Gel Electrophoresis for the detect... Gel -Stacking ger -Resolving gel Glass plate Buffer Electrophoresis Cathode Anode Figure 4: Summary of Gel electrophoresis Please watch the following youtube videos on SDS-PAGE. https://www.youtube.com/watch?v=i_6y6Z5UvwE https://www.youtube.com/watch?v=On_ZotdZexI Please answer the following questions in the space given below: 1. What are the two types of gels used in poly acrylamide gel electrophoresis? 2. What is the role of SDS in SDS-PAGE? 3. Why is the gel soaked in coomassie stain?
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Transcribed Image Text:Done Gel Electrophoresis for the detect...
Gel
—Stacking get
-Resolving gel
Glass plate
Buffer
↓
Electrophoresis
Cathode
Figure 4: Summary of Gel electrophoresis
Please watch the following youtube videos on SDS-PAGE.
https://www.youtube.com/watch?v=i_6y6Z5UvwE
https://www.youtube.com/watch?v=On_ZotdZexI
Please answer the following questions in the space given below:
1. What are the two types of gels used in poly acrylamide gel
electrophoresis?
2. What is the role of SDS in SDS-PAGE?
3. Why is the gel soaked in coomassie stain?
4. What are the building blocks of proteins?
5. If you have two proteins with masses of 25kDa and 40kDa,
which protein will travel farther in the gel in SDS-PAGE?
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