DNA fingerprinting uses to produce DNA fragments that can be separated and screened for the presence of genetic diseases. anticodons O bacterial plasmids O extracellular DNA O codons O restriction enzymes

Q: Which of the following functions in the regulation of gene expression?   plasmids euchromatin…

A: Gene expression in eukaryotic cells is mediated by activators and repressors of transcription as…

Q: Eco01091 2074 Pfol 46 BstAPI 170 Aatil 2017 Sspl 2501 Ndel 183 Ehel 235 Pdml 2204, Bogi 2215 Soal…

A: Restriction endonucleases also referred to as restriction enzymes are enzymes that cut DNA at…

Q: dye-linked substrate called X-gal (5-bromo-4-chloro-3-indolyl-ß-D-galacto-pyranoside) into galactose…

A: The lac operon contains a group of related genes (promoter, operator, Lac Z, Lac A, and lac Y) that…

Q: Deletions of entire genes from the chromosome can be repaired by:  Group of answer choices…

A: Introduction Deoxyribonucleic acid (DNA) is a polymer made up of two polynucleotide chains that coil…

Q: Which of the following DNA repair mechanisms relies on homologous DNA recombination for the repair…

A: DNA repair mechanisms relies on homologous DNA recombination for the repair processes are :  SoS…

Q: Why are R plasmids important?

A: Plasmid is an extrachromosomal self-replicating structure present in the bacterial cell. It carries…

Q: In order to construct a genome library, which statement most accurately describes the correct order…

A: Genomic DNA is an entity's chromosomal DNA that serves as a representation of the entirety of its…

Q: Plasmids are likely to contain

A: Plasmids are likely to contain:Antibiotic genes, A promoter associated with each gene, Restriction…

Q: Which of the following enzymes in E. coli synthesizes new DNA at sites of DNA damage during base…

A: The DNA repairing by the base excision repair and the nucleotide excision repair is done by the…

Q: Which of the following DNA repair mechanisms relies on homologous DNA recombination for the repair…

A: Homologous recombination repair is a DNA repair process that includes the invasion of an undamaged…

Q: During plasmid extraction, after the addition of acetate-containing neutralization buffer the large…

A: Plasmid extraction is a method for the isolation of DNA. In this method, purified plasmids are…

Q: Define the following words: replication fork, leading strand, lagging strand, Okazaki fragments,…

A: DNA replication is a biological process that occurs in all living organisms and is the basis for…

Q: Plasmids are the only vectors currently available for use in recombinant procedures.True or false?

A: The technology used for joining different DNA molecules from different species is known as…

Q: Multiple antibiotic resistance genes are contained on a bacterial plasmid in a __________.…

A: Introduction Bacteria are unicellular, prokaryotic organisms. They do not have true nucleus and…

Q: A plasmid provides its host with kanamycin resistance. What will happen to the plasmid if you…

A: Plasmids are defined as extrachromosomal circular genetic elements that are capable of…

Q: A commonly used plasmid that we will use in this lab is PUC19: (New England Biolabs) Indl 91 BamBI…

A: pUC19 is a circular double stranded DNA having 2686 base pairs.It is one of the most widely used…

Q: Base oxidation (small damage to a single nucleotide) is repaired using:

A: Introduction:DNA damage can occur due to various factors, including exposure to reactive oxygen…

Q: Jm109 cells transformed with ligation and plasmid alone is used. The colonies most like:   white…

A: β-galactosidase is an enzyme encoded by the lacZ gene and is used to break down lactose to glucose…

Q: The origin is the site on the DNA: is ONLY where transcription begins is ONLY where translation…

A: Origin is the site seen on the DNA sequence which initiates the process of replication. This is…

Q: I the following statements about genetic exchange in bacteria is NOT true? a Plasmids do not have to…

A: Most bacteria are haploid organisms means carrying only one copy of each gene per cell which usually…

Q: Why most genes conferring antibiotic resistance reside on R plasmids in pathogens associated with…

A: Antibiotic resistance is the surviving strategy used by the bacteria to escape antibiotics that are…

Q: What type of label is used for fluorescence in situ hybridization (FISH) probes?

A: Answer: Introduction: Fluorescence in situ hybridization (FISH) is a method performed in laboratory…

Q: E. coli Hfr cells can transfer genes to F cells. can form sex pili (F pili). typically do not…

A: A high-frequency recombination cell (Hfr cell) (also called an Hfr strain) is a bacterium with a…

Q: Explain the features of commercially prepared plasmids ?

A: Plasmid play a critical role , specially in the area of genetic engineering , such as gene cloning ,…

Q: How does restriction enzyme digestion allow for the determination of the correct plasmid construct…

A: Restriction enzymes are proteins that cut DNA at specific sequences. These enzymes are found in…

Q: Briefly describe two different methods for inserting foreign DNA into plasmids, giving the strengths…

A: The plasmid is typically a small circular deoxyribonucleic acid (DNA) strand in the cytoplasm of the…

Q: .Describe the method you would use to test for the uptake of a plasmid with a DNA insert

A: Introduction: The introduction of recombinant DNA into a suitable host cell is followed by the…

Q: A plasmid comprised of B-DNA changed its linking number from 420 to 457, resulting in a superhelix…

A: Introduction: DNA in the cell must be arranged in order to allow the packing of large DNA molecules…

Q: One of the frist steps in isolating plasmid DNA via mini-prep is to pellet the cells after O/N…

A: 1) The use of Resuspension buffer in the extraction or the isolation of the plasmid from the DNA is…

Q: Which of the following statements about genetic exchange in bacteria is NOT true? Oa Plasmids do not…

A: In the process of conjugation, the donor cell is dead and the recipient cell is alive

Q: In Polymerase Chain Reaction (PCR). Primers are short segments of DNA that. Obalance the pH of a…

A: Polymerase Chain Reaction or PCR is a molecular technique allowing the synthesis of multiple copies…

Q: We use a NanoDrop spectrophotometer to analyze plasmid DNA obtained from miniprep. What information…

A: A spectrophotometer is a device that measures the amount of light a solution has absorbed in order…

Q: Why are there strict reminders about mixing in the inverted position and not vortexing Plasmid…

A: A plasmid is a small, circular, double-stranded DNA molecule that is separate from the chromosomal…

Q: "Plasmids are still the workhorses for many applicationsof recombinant DNA technology". Define the…

A: Introduction  Microorganisms such as bacteria, viruses, etc. are not just pathogenic and harmful but…

Q: Categorize the following as being associated with transformation, conjugation, or transduction. Note…

A: There are three principal methods of genetic transfer between bacteria. Transformation deals with…

Q: Which of the following is directly responsible for catalyzing the synthesis of a complimentary…

A: *The answer of this question is option 3 that is Taq polymerase. *Taq polymerase isolated from…

Q: Which of the following repair mechanisms removes damaged bases in non-dividing cells?

A: Ans- Bae Excision Repair The common mechanism of DNA repair System is- Base Excision Repair (BER)–…

Q: Please help me this question ASAP need to submit urgently

A: Answer is option b.) double stranded , circular DNA molecule.

Q: Sequence the steps to transfect and observe a fluorescent protein in a slice of the brain:

A: Transfection is the process of artificially introducing nucleic acids (DNA or RNA) into cells,…

Q: Which statement about plasmid is FALSE: Plasmid is nonessential DNA in the bacteria Plasmids…

A: Plasmids are extrachromosomal DNA molecules found in some bacteria. They are typically small,…