Difference between streak plate method and serial dilution

Q: Determine what percentage of the culture was living (viable) and what percentage was dead…

A: The word "CFU" in microbiology refers to a colony-forming unit, which is used to estimate how many…

Q: student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step…

A: 5. The loop was flamed

Q: ext/map/ aining Lab Report Place the descriptions into the correct boxes to test your understanding…

A: Gram staining is a method frequently used to distinguish between two sizable groups of bacteria…

Q: You will only isolate pure colony to inoculate again (sub-culturing) to newly prepared plate medium,…

A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…

Q: If no amplified product was visualized by UV light on the gel, does it mean that  there is no…

A: Introduction :- In biology, a pathogen is any organism or agent that has the potential to cause…

Q: What is the difference between the pour plate method and the spread plate method? Why might you…

A: Standard plate count method is a growth based approach that counts the number of living…

Q: What is the physical appearance (solid, liquid, semi-solid) of following media: agar plate

A: The Agar plate is made from mixing agarose and a growth medium onto a petri dish. Agarose is in a…

Q: Which of the following serves as a counterstain for the gram-stain technique? It also happens to be…

A: Gram staining is a technique used to differentiate two groups of bacteria based on the different…

Q: Both the Endospore Stain Protocol and Mannitol Salt Agar Media are differential. How can this be so?

A: The growth media are nutritious media that are used to grow microbes (bacteria and fungus) in the…

Q: What is the purpose of a streak plate and how do you know one was successful? Explain.

A: In microbiology, the Streak plate method is used to get pure isolated colonies. As we know that a…

Q: Remove the agar plate from its lid with the left hand. While holding the plate, inoculate the agar…

A: Introduction -- Microbes are around and every where in environment. Sterile agar plates which…

Q: Why is it best to use sterile distilled water in the preparation of microbial suspension and dry…

A: Smear preparation is the first step carried out during the staining procedure.

Q: Why is it important to use a sterilized loop between streaks when preparing a streak­plate?…

A: Streak plates are prepared to grow and isolate individual colonies of bacteria. Here, we will mark…

Q: Explain the reason for the effect on mass spectroscopy method of diagnosis by culture conditions.

A: Mass Spectrometry is an analytical method. It is used to quantify known materials to identify…

Q: In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable…

A: Harada-Mori culture technique It is a method of incubating fecal material on a filter paper strip in…

Q: Which of the following is not meant to reduce contamination of yoru sample? avoiding hand contact…

A: A microbial culture is done to multiply the specific strains of the microorganisms under specific…

Q: What is the description of the growth and growth pattern of the microorganisms in the spread plate…

A: -A quadrant or T streak plate is used to maintain or obtain a pure culture of a bacterium. A type of…

Q: For preparation of a smear on a slide, what is the purpose of heat fixation? What problems can arise…

A: The preparing a slide that has to be observed under a microscope there are multiple steps that are…

Q: Why is it necessary to perform a plate count in conjunction with the turbidimetry procedure?

A: Plate counting is one of the most fundamental techniques used in microbiology. It is used to…

Q: Why is 15 minutes generally used for sterilization? Why is agar added to medium?

A: Sterilization is the process in which all life forms like microbes (fungi, bacteria, viruses,…

Q: why In spread-plate technique, the bacterial suspension volume  should not exceed 0.1ml.

A: Spread plate method is a technique which uses a Petri plate or dish in which a growth medium or gar…

Q: After performing a Gram stain on a Gram negative bacteria, the specimen does not show pink or purple…

A: steps that are included in the Gram staining are- applying a primary stain (crystal violet) to a…

Q: A patient has subcutaneous nfection and the specimen is submitted on a swab . Explain why this is an…

A: Several biological tests involves testing of the the samples from the infected individuals body.…

Q: Identify the method of sterilization/ chemical agent described This chemical agent is used as a…

A: Various methods of sterilization have been used in laboratories and pharmaceutical industry.

Q: Define the following terms; Sterilization, and Aseptic Technique. What are the differences between…

A: Microbes invading and proliferating within an organism's body causes infection. Microbes include…

Q: but de prod the p ing hacterial clies that appear colrle with ak Salmonello-Shigell (SS) agar plate…

A: Salmonella shigella agar is a selective medium used for identification, isolation, cultivation…

Q: What are the possible reasons why culture plates may have too many colonies despite performing…

A: There are several possible reasons why culture plates may have too many colonies despite performing…

Q: What was the concentration of bacteria (CFU/mL) in the original suspensions used for dilutions and…

A: The ideal colony size is found between 30 and 300. The CFU/ml is calculated by: (Colony × Dilution…

Q: How can the thickness of the smear affect the staining procedure?

A: Smear can e defined as the spread of cells in a layer to observe it under microscope. Since, cells…