Determine the number of carbon atoms present and the the total number of phosphate groups present in each of these following intermediates of glycolysis: a. Glyceraldehyde 3-phosphate b. 3-Phosphoglycerate
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Determine the number of carbon atoms present and the the total number of phosphate groups present in each of these following intermediates of glycolysis:
a. Glyceraldehyde 3-phosphate
b. 3-Phosphoglycerate
Step by step
Solved in 2 steps
- The Glycolytic pathway describes the oxidation of glucose to pyruvate with the generation of ATP and NADH Which of the following 8 statements are correct about the process of Glycolysis? a.Dihydroxyacetone phosphate is isomerized to the aldehyde glyceraldehyde 3-phosphate b. phosphoenolpyruvate is formed from the loss of a hydrogen atom on carbon 2 and a hydroxyl group from carbon 3 c. Glucose transporters only transports phosphorylated glucose d. 2 molecules of NADH is produced from the phosphorylated oxidation of Glyceraldehyde-3-phosphate e. when phosphoenolpyruvate is converted to pyruvate, 1 molecule of ATP is produced f. Fructose-1,6.biphosphate is cleaved by aldolase to form 2 molecules of glyceraldehyde-3-phosphate g. The substrate level phosphorylation of 1,3-biphosphoglycerate yields 2 molecules of ATP h. Glyceraldehyde-3-phosphate loses a hydrogen atom on carbon 1 and gains an oxygen atom i. The ketose sugar glucose-6-phosphate is…Specify the number of carbon atoms present and the number of phosphate groups present in each of the following glycolysis intermediates (3 points each) 1. Glucose 6 phosphate 2. Fructose 1, 6, phosphate 3. Glyceraldehyde 3-phosphate 4. 3-phospholglycerate 5. Fructose 6 phosphateDetermine the number of carbon atoms present and the the total number of phosphate groups present in each of these following intermediates of glycolysis: a. Glucose 6-phosphate b. Fructose 1,6-phosphate
- In the first stage of glycolysis, fructose-1,6-bisphosphate iscleaved to form glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. The latter molecule can then be converted to glyceraldehyde-3-phosphate. Illustrate themechanisms whereby these reactions occurWhich of the following is a correct ranking of molecules with respect to their energy value in glycolysis (note: >means “greater than”)? (a) two pyruvates > one glucose (b) one glucose > one fructose-1,6-bisphosphate (c) twoglyceraldehyde-3-phosphates (G3P) > one glucose (d) two pyruvates > one fructose-1,6-bisphosphate (e) two pyruvates > two glyceraldehyde-3-phosphates (G3P)Indicate the general type of enzyme that mediates each glycolysis reaction depicted below. (e.g. Transferase, Oxidoreductase, Kinase, Hydrolase, Lyase, or Isomerase) iii. O H Glyceraldehyde-3-phosphate → 1,3-Bisphosphoglycerate H-C-OH + NAD CH₂O-P + P₁ H-O- -C-H Glucose →→ Glucose-6-phosphate H b 0 OH HO OH H-C- 0 -H HO- H ОН H-C-OH + NADH +H+ CH₂O P P ATP ADP 1 H (P-O- -C- H H HO OH 2-Phosphoglycerate → Phosphoenolpyruvate 0 OH H ОН -O~(P) + HOH
- During gluconeogenesis, the three irreversible steps of glycolysis have to be bypassed. The first step is the conversion of pyruvate to phosphoenolpyruvate. Which of the following statement is false regarding the reaction step? Select one: a. Conversion of oxaloacetate from pyruvate occurs in mitochondria and shuttled into the cytosol. b. Formation of phosphoenolpyruvate requires both ATP and GTP as an energy source. c. Acetyl CoA is an activator of enzyme pyruvate carboxylase. d. This reaction involves two-step process catalyzed by pyruvate carboxylase and phosphoenolpyruvate carboxykinaseIf 32P-labeled ATP is provides for the reactions of glycosis, which of the following would be TRUE about 1.3 biphosphoglycerate? A. 50% of 1,3-bis phosphoglycerate would contain 32P B. 100% of 1,3-bis phosphoglycerate would contain 32P on carbon 1 C. 100% of 1,3-bis phosphoglycerate would contain 32 P on carbon 3 D. 50% of 1,3-bis phosphoglycerate would contain 32P on carbon 3 E. 50% of 1,3-bis phosphoglycerate would contain 32P on carbon 1The glucose/glucose-6-phosphate substrate cycle involves distinct reactions of glycolysis and gluconeogenesis that interconvert these two metabolites. Assume that under physiological conditions, [ATP] = [ADP]; [P;] = 1 mM. Consider the glycolytic reaction catalyzed by hexokinase: ATP + glucose ADP + glucose-6-phosphate AG = - 16.7 kJ/mol (a) Calculate the equilibrium constant (K) for this reaction at 298°K, and from that, calculate the maximum [glucose-6-phosphate]/ Iglucose] ratio that would exist under conditions where the reaction is still thermodynamically favorable. (b) Reversal of this interconversion in gluconeogenesis is catalyzed by glucose-6-phosphatase: glucose-6-phosphate + H20 = glucose + P AG" = -13.8 kJ/mol
- In which of the following metabolic conversions is ATP “generated” during glycolysis? Phosphoenolpyruvate -> pyruvate 2-Phosphoglycerate —> Phosphoenolpyruvate Glucose-6-phosphate —> Fructose-6-phosphate Fructose-1,6-bisphosphate —> Dihydroxyacetone phosphate + Glyceraldehyde-3-phosphate Fructose-6-phosphate —> Fructose-1,6-bisphosphateWhich of the following statements are True as to the location in pyruvate of labeled carbons if glucose molecules labeled (in separate experiments) with 14C (radioactive carbon) at each position of the carbon skeleton proceed through the Glycolytic pathway. Multiple answers:Multiple answers are accepted for this question a The carboxyl carbon of pyruvate derives from carbon 3 of glucose. b The keto carbon of pyruvate derives from carbon 2 of glucose. c The methyl carbon of pyruvate derives from carbon 1 of glucose. d The carboxyl carbon of pyruvate derives from carbon 4 of glucose. e The keto carbon of pyruvate derives from carbon 5 of glucose. f The methyl carbon of pyruvate derives from carbon 6 of glucose. g The carboxyl carbon of pyruvate derives from carbon 2 of glucose. h The keto carbon of pyruvate derives from carbon 3 of glucose. i The methyl carbon of pyruvate derives from carbon 4 of glucose.The glucose/glucose-6-phosphate substrate cycle involves distinct reactions of glycolysis and gluconcogenesis that interconvert these two metabolites. Assume that under physiological conditions, [ATP] = [ADP] and [Pi] =1 mM. Consider the following glycolytic reaction catalyzed by hexokinase: ATP + glucose = AG' = -16.7 kJ/mol ADP + glucose-6-phosphate (a) Calculate the equilibrium constant (K) for this reaction at 298 K, and from that, calculate the maximum [glucose-6-phosphate]/[glucose] ratio that would exist under conditions where the reaction is still thermody- namically favorable. (b) The reverse of this interconversion in gluconeogenesis is catalyzed by glucose-6-phosphatase: glucose-6-phosphate + H,0 = glucose + P, AGr = -13.8 kJ/mol K= 262 for this reaction. Calculate the maximum ratio of [glucose]/ [glucose-6-phosphate] that would exist under conditions where the reaction is still thermodynamically favorable. (c) Under what cellular conditions would both directions in the…