design a analog of dna intercalator which has electrostatic interactions with DNA and crosslinks with dna. keep the oxygens in final product. use the provided structure to build
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design a analog of dna intercalator which has electrostatic interactions with DNA and crosslinks with dna. keep the oxygens in final product. use the provided structure to build
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- Give the name of the enzyme that catalyzes each of the following reactions:(a) Makes a DNA strand from a DNA template. (b) Makes a DNA strand from an RNA template. (c) Makes an RNA strand from a DNA templateMake the complementary strand for the following DNA template and label both strands as 5’ to 3’ or 3’ to 5’ (hint: determine first if P = phosphate or –OH are the 5’ or 3’ end of the strand). Draw an arrow showing the direction of synthesis of the new strand. How many total hydrogen bonds are in this double strand of DNA? Template : P- AGACTTG-OH New strand :Make the complementary strand for the following DNA template and label both strands as 5’ to 3’ or 3’ to 5’ (hint: determine first if P = phosphate or –OH are the 5’ or 3’ end of the strand). Draw an arrow showing the direction of synthesis of the new strand. How many hydrogen bonds are in this double strand of DNA? Template : P- AGGCTCG-OH New strand :
- Consider the structure of double-stranded DNA. When DNA is placed into distilled water, it denatures; however, by adding NaCl, the DNA renatures. Why?Draw the reaction between a growing strand of RNA and the next nucleotide added. Clearly show the interaction between the template DNA and the rNTP and draw arrows to indicate the reaction that occurs. How does this compare to what occurs in DNA polymerization?Choose reactions that always require hydrolysis of ATP. Select all that apply. sliding along template strands unwinding of DNA strands by helicase formation of the phosphodiester linkage by DNA ligase unwinding of DNA strands by B2 subunits formation of the phosphodiester linkage by DNA polymerase I
- During agarose gel electrophoresis, why does DNA move through the gel when electric current is applied? because DNA is negatively charged because a charged chemical from the loading buffer is bound to the DNA because DNA is positively charged because DNA absorbs electricityDetermine what amino acid will be formed from the given DNA strand below: 3’ T A C A T G C C G A A T 5’ Note: Prepare the partner strand of this DNA. Discuss how will replication happen by mentioning the enzyme needed then transcribe to form mRNA. Discuss what will happen to mRNA, then translate, mentioning the anticodon to be used. Look at the genetic code to know what amino acid will become part of the polypeptide chain. 1. Partner DNA strand 2. the mRNA strand 3. the tRNA 4. the formed amino acidsExplain how cells activate nucleic acids for polymerization. Explain why DNA is stable and why its structure dictates its replication mechanism. Explain why many RNA molecules exhibit tertiary structure, while most DNA molecules do not. Explain how DNA replication occurs from structural and enzymatic perspectives. Develop an understanding of nucleic acid biology outside a natural biological context (such as PCR, etc.)
- Given the active site diagram below, please identify the residue participating in a charge-charge interation. HO OH HN OH 5 ΝΗ *HN ΝΗ OH O Zn²+ 2 4 5 3 1 2 NH 3Compare and contrast the properties of DNA polymerase and RNA polymerase. Drag the appropriate items to their respective bins. can proofread using a 3'-to-5' exonuclease activity polymerize in a 5'-to-3' direction Only RNA can initiate strand synthesis catalyze phosphodiester bond formation to polymerize nucleotides into nucleic acids Only DNA use deoxyribonucleotide triphosphates as substrates can only extend an existing strand Both Reset Help dependent on a DNA sequence template use ribonucleotide triphosphates as substratesShow the structure of a DNA where the lead strand is ATCG. Show H-, glycosidic, phosphoester bondings.
