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Describe the mesomeric or resonance effect and differentiate between types +E or +M and -R or -M.
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- (A) You measured the absorbance of a solution of 0.2 mM NADH at 340 nm and the reading was 0.55. Calculate the extinction coefficient of NADH.What is the molecular prbital diagram for H22+ ?(2) Adenine has a molar absorbance of 4 13.1 M-1cm-1 for 263 nm nucleic acid. What is the molar concentration of an acid solution that shows a permeability of 75% when 2 ml of it is placed in an absorption cell with a thickness of 1 cm?
- COSY is one of 2D spectras which are widely used for the structure determination of proteins with a mass of up to 10 kD. What gives rise to cross signals in a COSY experiment?(b) The formula that relates the spin only magnetic moment (en) to the number of unpaired d-electrons (x) is: Heft = √√x(x+2) (1) For each of the following complexes, use this formula to calculate the number of unpaired electrons: Nan[Rh(CO)4], Hell0 BM Rb₂lMoBrn), Heft=2.83 BM (ii) Using the answers from part (b)(i): • Draw crystal field d-orbital energy level diagrams for each complex. • State the oxidation state of the metal in each complex. • Determine the value of n for each complex.(17,18-3,3) Select the most acidic hydrogen in the molecule below.
