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- Describe the limitations of FANA
- Describe the other Assays for ANA testing
- What are the advantages of FANA over the other assays?
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- Bradford Assay Fill in the average A595 and A595 of sample minus A595 of blank. What to do? Calculating the average absorbance of the standards Subtracting the absorbance of the blank (the sample with 0 g/ml) from the absorbance of the other samples Concentration (micrograms/ml) A595 - trial 1 A595 - trial 2 A595 - trial 3 average A595 A595 of sample minus A595 of blank 0 1.501 1.446 1.447 2 1.624 1.558 1.559 5 1.731 1.749 1.712 10 1.901 1.838 1.892 15 2.161 2.108 2.228 18 2.231 2.277 2.319Briefly describe the principle of the MTT assay and how this assay helps youdetermine the LC50 of drug X.1.a)What is the equivalence point and how does it relate to the recommended proportion of serum to blood in the heme agglutination assay? b. what would the predicted outcome be if you used too little serum in this assay? Why? c. what would the predicted outcome be if you used too much serum in assay? Why?
- Bradford Assay Fill in the average A595 and A595 of sample minus A595 of blank. Show some sample computation. Concentration (micrograms/ml) A595 - trial 1 A595 - trial 2 A595 - trial 3 average A595 A595 of sample minus A595 of blank 0 1.501 1.446 1.447 2 1.624 1.558 1.559 5 1.731 1.749 1.712 10 1.901 1.838 1.892 15 2.161 2.108 2.228 18 2.231 2.277 2.319Explain the basic principles of Elispot AssayBriefly compare the following test systems, Micro-tox, ATP-Tox, and Daphnia magna IQ test . Explain how each assay works (the principle), not the test procedure.
- List some conditions that cause false positive reactions with the RPR test?Which of the following statements is/are FALSE about Bradford assay? 1. Upon addition of the reagent, the color of the protein solution becomes purple. II. The absorbance of the resulting solution is measured at a wavelength of 540-550 nm. Both I and II O II only Neither I nor II O I onlyCalculation for a neutral red assay please include steps since it is essential for the report and it is marked.
- Explain the source of error sources of error- in relation to the assay, errors can be from extraction method or the assay itself (like use of spectro), explain the effect of the error especially to the absorbanceAfter adding stop solution (sulphuric acid) in reaction mixture of HRP assay with TMB, yellow color becomes brighter. What is the mechanism?With detail, compare and contrast the following 5 real-time assays; Taqman, SYBR Green, Molecular Beacon, FRET, and Scorpion. Please write complete sentences in paragraph form. Identify how the assays are similar but more importantly, what are the distinguishing features of each of the technologies.