DCEC e + + Rh-hr + SC1 + SC2 + 0 + SC3 0 0 0 + ID1 0 0 ID2 + ID3 + ID4 0 ID5 0 0 ID6 0 0 ID7 0 ID8 + 0 ID9 0 0 ID10 00 ID11 0 0 0 + AC AC, auto control; SC, screening cells; ID, identification panel; Neg. Negative reaction O + + 0 0 0 0 + 0 0 0 + 0 + 0 + 0 + + + 0 + + + + + 0 0 + O + 0 O 0 0 0 0 0 0 0 0 0 0 0 + 0 + + 0 Kell Duffy Kidd Lewis MNS Luth. k Kp Kp Js Js Fy Fy Jk JK Le Le P1 MNS S Lu Lu 00 + + + + + + + + 00 0 + 00+ 0 0 0 0 0 0 0 0 0 + 0 + O + 0 0 0 0 0 0 0 0 + 0 + + 0 + + 0+ 0 + + + + + + + + 0 0 0 0 + 0 0 0 0 + + 0 + 0 + 0 + + 0 O + 0 + 0 0 + + 0 + 0 0 + 0 + 0 + + 0 0 + + 0 0 O 0 0 0 0 + 0 0 + + 0 + + + 0 + 0 0 + 0 0 + + 0 + + + + 0 0 0 + + 0 0 + ● 0 0 0 + 0 0 + 0 0 + + 0 + + + + 0 0 + + + 0 0 0 0 0 0 0 + + + 0 + 0 + + + + + + 0 + 0 + 0 + + + + + Xg Results Xg AHG 0.2 M DTT +2+ 2+ + Neg Neg + 2+ Neg 2+ +2+ 2+ 0 Neg Neg +2+ 2+ Neg Neg 0 2+ Neg +Neg Neg + Neg Neg 0 Neg Neg 0 Neg Neg + Neg Neg Neg Note: SC1 to SC3 are screening cells, ID1 to ID11 are panel cells. All screening and panel cells have negative results with the IS phase and Thermo Phase. 1. What is the result of the Antibody Screening test? 2. Which of the following panel cells contain only the Js antigen? 3. Based on the result of the autocontrol, it is safe to say that the antibodies which reacted with the screening cells and panel cells are: 4. Which of the following antigens on the screening and panels cells were destroyed by 0.2M DTT (dithiothreitol)?
DCEC e + + Rh-hr + SC1 + SC2 + 0 + SC3 0 0 0 + ID1 0 0 ID2 + ID3 + ID4 0 ID5 0 0 ID6 0 0 ID7 0 ID8 + 0 ID9 0 0 ID10 00 ID11 0 0 0 + AC AC, auto control; SC, screening cells; ID, identification panel; Neg. Negative reaction O + + 0 0 0 0 + 0 0 0 + 0 + 0 + 0 + + + 0 + + + + + 0 0 + O + 0 O 0 0 0 0 0 0 0 0 0 0 0 + 0 + + 0 Kell Duffy Kidd Lewis MNS Luth. k Kp Kp Js Js Fy Fy Jk JK Le Le P1 MNS S Lu Lu 00 + + + + + + + + 00 0 + 00+ 0 0 0 0 0 0 0 0 0 + 0 + O + 0 0 0 0 0 0 0 0 + 0 + + 0 + + 0+ 0 + + + + + + + + 0 0 0 0 + 0 0 0 0 + + 0 + 0 + 0 + + 0 O + 0 + 0 0 + + 0 + 0 0 + 0 + 0 + + 0 0 + + 0 0 O 0 0 0 0 + 0 0 + + 0 + + + 0 + 0 0 + 0 0 + + 0 + + + + 0 0 0 + + 0 0 + ● 0 0 0 + 0 0 + 0 0 + + 0 + + + + 0 0 + + + 0 0 0 0 0 0 0 + + + 0 + 0 + + + + + + 0 + 0 + 0 + + + + + Xg Results Xg AHG 0.2 M DTT +2+ 2+ + Neg Neg + 2+ Neg 2+ +2+ 2+ 0 Neg Neg +2+ 2+ Neg Neg 0 2+ Neg +Neg Neg + Neg Neg 0 Neg Neg 0 Neg Neg + Neg Neg Neg Note: SC1 to SC3 are screening cells, ID1 to ID11 are panel cells. All screening and panel cells have negative results with the IS phase and Thermo Phase. 1. What is the result of the Antibody Screening test? 2. Which of the following panel cells contain only the Js antigen? 3. Based on the result of the autocontrol, it is safe to say that the antibodies which reacted with the screening cells and panel cells are: 4. Which of the following antigens on the screening and panels cells were destroyed by 0.2M DTT (dithiothreitol)?
Chapter5: Unit, Percentage, Milliequivalent, Ratio, And Household Measures
Section: Chapter Questions
Problem 1.3P
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Question
Transcribed Image Text:Rh-hr
Kell
Duffy
Xg Results
D C E с
Kp Kp Js Js Fy Fy
Xg AHG 0.2
M
DTT
+
+
+
0 +
+ 2+ 2+
+ 0
0 0
0
+
0
+
0
0
0
0
+ Neg Neg
0
0
+ 0
+
+
0
+
0
0
+
+2+ Neg
+2+ 2+
+
+
+
+
0
+
0
+
0
0 +
+
0
+
+
0
+
0
+
0
+
0
+2+ 24
+
0
0
0
0
+
0
+
0
0
+
+
0 Neg Neg
ID4
0 +
+
0
0
0
+
0
+ 0
+
+2+ 2+
Neg Neg
ID5
0 0
+
0
0
+
0
+
+
0
+ 0
+
+
ID6
0 0
+
0
+
+
0
0
0
0
+
+
+
0
+
0 2+ Neg
ID7
0 0
+
0
0
+
0
0
0
+
0
0 0
0
+
0
0
+
+ Neg Neg
ID8
+ 0
+ +
0
0 +
0
+
0 0
+
0
0
+ 0
+
+
0
+
+ Neg Neg
ID9
0 0
+
0
0
0
+
0
0
+
+
0
0
0
+ 0
+
+
+
0 Neg Neg
ID10 000
+
0
0
+
+
+ 0
0
0
+
0
+
+
0
+
0
+
0 Neg Neg
ID11
000
+
00+
0+ 0
0
+
0 +
0
+
0 + 0
0 +
+Neg Neg
AC
Neg
AC, auto control; SC, screening cells; ID, identification panel; Neg. Negative reaction
Note: SC1 to SC3 are screening cells, ID1 to ID11 are panel cells. All screening and panel cells have negative results with the IS phase and Thermo
Phase.
1. What is the result of the Antibody Screening test?
2. Which of the following panel cells contain only the Js" antigen?
3. Based on the result of the autocontrol, it is safe to say that the antibodies which reacted with the screening cells and panel cells are:
4. Which of the following antigens on the screening and panels cells were destroyed by 0.2M DTT (dithiothreitol)?
5. The antibodies identified using the panel cells above belongs to what class of immunoglobulins?
6. Is matching pattern achieved for all of the identified antibodies?
7. One of the identified antibody using these panel cells is anti-K. Is the rule of 3 achieved for anti-K?
8. Aside from anti-K, what other antibodies are also probably present based on the results of the antibody panel above?
9. Give possible Rh genotype/s (using Weiner nomenclature) for SC1.
10. Give possible Rh genotype/s (using Weiner nomenclature) for ID1.
SC1
SC2
SC3
ID1
ID2
ID3
O
O
+ +
+
0
00
0
+
0
+
00
00
O
+
a
3
+
O
+
+
+
+
00
+
+
+
+
+
+
+
+
+
O
+
Kidd
Jk Jk
+
+
0 +
0 0
0
+
+
+
0
0
+
+ 0
+
0
0
+
+
O
+
0
Lewis
Le Le
+
+
O
+
+
+
+
+ 0
O
P1
+
+
MNS
M NS
+
0
+
0
O
Luth.
S Lu Lu
+
+
+
+
+
O
+
0
+
+
0
0
0
0
00
0
+
+
+
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