d. You are given a mixture of three compounds (A, B, C, below). он CH, осн, в A a. Rank the three compounds from least to most polar. A в least polar most polar CHM 2070 Lab Fall 2020 b. You conduct a TLC experiment on this mixture and obtain the TLC plate shown below on the left. Identify all three compounds on the TLC plate

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**Educational Text and Explanation:**

**CHM 2070 Lab - Fall 2020**

**Compound Analysis and Chromatography**

**d.** You are given a mixture of three compounds (A, B, C, as shown below):

- Compound A: Naphthalene derivative with a methyl group (CH₃)
- Compound B: Naphthalene derivative with a hydroxyl group (OH)
- Compound C: Naphthalene derivative with a methoxy group (OCH₃)

**a.** **Rank the three compounds from least to most polar:**

- A (least polar) → C → B (most polar)

---

**b.** You conduct a Thin Layer Chromatography (TLC) experiment on this mixture and obtain the TLC plate shown on the left. Identify all three compounds on the TLC plate:

- The TLC plate is divided into three columns representing different solvent polarities.
- The left column is with the original solvent showing three distinct spots at varying heights.

**c.** **What would the TLC plate look like if you used a less polar solvent?**

- In the middle column ("less polar solvent"), the spots would be lower on the plate compared to the original as the compounds do not move as much.

**d.** **What would the TLC plate look like if you used a more polar solvent?**

- In the right column ("more polar solvent"), the spots would be higher on the plate as the compounds travel further with a more polar solvent.

**e.** **Calculate the Rf value of the middle spot of the first plate.**

- Use a ruler to measure the distance traveled by the middle spot and divide it by the total distance traveled by the solvent front to calculate the Rf value. (Rf = distance traveled by compound / distance traveled by solvent front)

**f.** **In what order would the three compounds elute in column chromatography?**

- **Order to elute:**
  - First to elute: A
  - Middle: C
  - Last to elute: B

This activity demonstrates the use of TLC to determine the polarity of compounds and the effect of solvent polarity on the separation process. Understanding the order of elution is crucial for effective separation and purification in column chromatography.
Transcribed Image Text:**Educational Text and Explanation:** **CHM 2070 Lab - Fall 2020** **Compound Analysis and Chromatography** **d.** You are given a mixture of three compounds (A, B, C, as shown below): - Compound A: Naphthalene derivative with a methyl group (CH₃) - Compound B: Naphthalene derivative with a hydroxyl group (OH) - Compound C: Naphthalene derivative with a methoxy group (OCH₃) **a.** **Rank the three compounds from least to most polar:** - A (least polar) → C → B (most polar) --- **b.** You conduct a Thin Layer Chromatography (TLC) experiment on this mixture and obtain the TLC plate shown on the left. Identify all three compounds on the TLC plate: - The TLC plate is divided into three columns representing different solvent polarities. - The left column is with the original solvent showing three distinct spots at varying heights. **c.** **What would the TLC plate look like if you used a less polar solvent?** - In the middle column ("less polar solvent"), the spots would be lower on the plate compared to the original as the compounds do not move as much. **d.** **What would the TLC plate look like if you used a more polar solvent?** - In the right column ("more polar solvent"), the spots would be higher on the plate as the compounds travel further with a more polar solvent. **e.** **Calculate the Rf value of the middle spot of the first plate.** - Use a ruler to measure the distance traveled by the middle spot and divide it by the total distance traveled by the solvent front to calculate the Rf value. (Rf = distance traveled by compound / distance traveled by solvent front) **f.** **In what order would the three compounds elute in column chromatography?** - **Order to elute:** - First to elute: A - Middle: C - Last to elute: B This activity demonstrates the use of TLC to determine the polarity of compounds and the effect of solvent polarity on the separation process. Understanding the order of elution is crucial for effective separation and purification in column chromatography.
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