Create a Benesi-Hildebrand Plot. To do this, plot the data of 1/(F-F0) (y-axis) vs.1/[CD]0 (x-axis). Fit the data to [Egn 8]. The fitting parameters of the data to astraight-line equation (y = mx + b) should allow you to calculate Ka. Take carewith units, concentrations will need to be M when reporting the Ka value.X ul of ME-b-CD(20mM)[ME-b-CD]ul of ANSY ul ofFluorescence(mM)(0.4mM)PBS[CD] mMintensityF- FO50015000.3710050014009.4269.05622005001300216.7616.393.30050012003.22.62122.2514005001100427.49627.126500500100030.57330.2036.60050090034.34433.9747700500800736.99136.6218.8005007008.38.47838.1089.9005006009.41.01240.6421010005005001043.76943.3991212005003001245.6445.271414005001001447.5247.151515005001548.35947.989

Answered: Image /qna-images/answer/5b9db3e7-aee4-419c-ba15-577c1c3bed94.jpg

Create a Benesi-Hildebrand Plot. To do this, plot the data of 1/(F-F0) (y-axis) vs. 1/[CD]O (x-axis). Fit the data to [Egn 8]. The fitting parameters of the data to a straight-line equation (y = mx + b) should allow you to calculate Ka. Take care with units, concentrations will need to be M when reporting the Ka value. X ul of ME-b-CD (20mM) [ME-b-CD] ul of ANS Y ul of Fluorescence (mM) (0.4mM) F- FO PBS [CD] mM intensity 500 1500 0.37 1 100 500 1400 1 9.426 9.056 200 500 1300 2 16.76 16.39 3. 300 500 1200 3. 22.621 22.251 400 500 1100 27.496 27.126 500 500 1000 30.573 30.203 6. 600 500 900 34.344 33.974 7 700 500 800 7 36.991 36.621 8. 800 500 700 8. 38.478 38.108 900 500 600 9. 41.012 40.642 10 1000 500 500 10 43.769 43.399 12 1200 500 300 12 45.64 45.27 14 1400 500 100 14 47.52 47.15 15 1500 500 15 48.359 47.989

Create a Benesi-Hildebrand Plot. To do this, plot the data of 1/(F-F0) (y-axis) vs. 1/[CD]O (x-axis). Fit the data to [Egn 8]. The fitting parameters of the data to a straight-line equation (y = mx + b) should allow you to calculate Ka. Take care with units, concentrations will need to be M when reporting the Ka value. X ul of ME-b-CD (20mM) [ME-b-CD] ul of ANS Y ul of Fluorescence (mM) (0.4mM) F- FO PBS [CD] mM intensity 500 1500 0.37 1 100 500 1400 1 9.426 9.056 200 500 1300 2 16.76 16.39 3. 300 500 1200 3. 22.621 22.251 400 500 1100 27.496 27.126 500 500 1000 30.573 30.203 6. 600 500 900 34.344 33.974 7 700 500 800 7 36.991 36.621 8. 800 500 700 8. 38.478 38.108 900 500 600 9. 41.012 40.642 10 1000 500 500 10 43.769 43.399 12 1200 500 300 12 45.64 45.27 14 1400 500 100 14 47.52 47.15 15 1500 500 15 48.359 47.989

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter17: Applications Of Infrared Spectrometry

Section: Chapter Questions

Problem 17.6QAP

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