Construct a calibration curve for the electrode, and report (a) the range of concentrations in which a linear response is observed, (b) the equation for the calibration curve in this range, and (c) the concentration of penicillin in a sample that yields a potential of 138 mV.

Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
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Mifflin and associates described a membrane electrode for the quantitative analysis of penicillin
in which the enzyme penicillinase is immobilized in a polyacrylamide gel that is coated on a glass
pH electrode. The following data were collected for a series of penicillin standards.
[Penicillin]
Potential
(M)
1.0x10-2
(mv)
215
2.0x103
199
1.0x10-3
2.0x10-4
185
148
1.0x104
130
1.0x10-5
91
1.0x10-6
75
Construct a calibration curve for the electrode, and report
(a) the range of concentrations in which a linear response is observed,
(b) the equation for the calibration curve in this range, and
(c) the concentration of penicillin in a sample that yields a potential of 138 mV.
Transcribed Image Text:Mifflin and associates described a membrane electrode for the quantitative analysis of penicillin in which the enzyme penicillinase is immobilized in a polyacrylamide gel that is coated on a glass pH electrode. The following data were collected for a series of penicillin standards. [Penicillin] Potential (M) 1.0x10-2 (mv) 215 2.0x103 199 1.0x10-3 2.0x10-4 185 148 1.0x104 130 1.0x10-5 91 1.0x10-6 75 Construct a calibration curve for the electrode, and report (a) the range of concentrations in which a linear response is observed, (b) the equation for the calibration curve in this range, and (c) the concentration of penicillin in a sample that yields a potential of 138 mV.
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