Construct a calibration curve by plotting the log10 (fragment size) against the distance migrated (in mm). Data is given in the picture to help plot the graph. Use excel for it and give appropriate titles to the graph.

Q: a. What are the purposes of sequence alignment?

A: Sequence alignment is a method by which one can arrange sequences of DNA, RNA and protein so as to…

Q: Calculate the volume (in µL) of DNA template required to add 60.0 ng to a 20.0 µL PCR if the DNA…

A: Concentration is the term used to define the amount of a specific entity with respect to the total…

Q: An AGE run was set at 100V for 30 min, where 3 ul of the ladder (100 ng/ul) was loaded into the gel,…

A: if 3ul of DNA ladder is added on the gel AGE which has a concentration of 100ng/ ul. Then it means :…

Q: This BLAST program performs the greatest number of searches for the same query sequence and database…

A: BLAST is used in bioinformatics an an algorithm which compares the sequences of amino acids and of…

Q: How  big (in base pairs)  is (are) the fragments generated by digesting the vector with EcoRI

A: Base pairs - there are four bases or nucleotides in DNA: ADENINE, CYTOSINE, GUANINE, and thymine.

Q: Calculate the DN/DS ratio for the sequences below. TTT ACT AAT AGT TTC CCT AAC GAT

A: A codon is a combination of three nucleotides that codes for an amino acid. A single codon codes…

Q: a PCR-based crime scene investigation, similar to the one presented in the lab module with Brother Y…

A: DNA sample taken from a crime scene is compared with a DNA sample from a suspect. If the two DNA…

Q: hat are the steps in creating a dna profile

A: DNA profiling is also known as DNA fingerprinting. It is often used to identify the origin and…

Q: This reflects the highest identification of the aligned sequences in the database to the sequence…

A: Aligned sequence refers to the sequence that is matched to the sequence present in the database.…

Q: Create your own flowchart on the process of rDNA technology

A:

Q: A student is trying to add 15.0 ng of DNA template to a 20.0 µL PCR.  The DNA template is at a…

A: Polymerase Chain Reaction or PCR is a method to make multiple copies of DNA starting from a small…

Q: Saenger’s DNA sequencing procedure. Include the method and the main reagents used

A: Its a DNA sequencing method

Q: Describe the process of doing a microarray.

A: A laboratory tool is used to measure the expression of thousands of genes at the same time and this…

Q: Sanger Illumina PacBio Amount of DNA needed for sequencing Read length Amount of data sequenced…

A: DNA sequencing DNA sequencing involves various techniques by which the order of nucleic acid…

Q: This descriptor refers to the highest alignment score of all matched sequences to the sequence…

A: The highest alignment score is determined by the BLAST algorithm and is based on the number of…

Q: Identify the structure indicated by the Yellow arrow.

A: The human body is a well built machine in which all the organs coordinate together to make it work.…

Q: An AGE run was set at 100V for 30 min, where 3 ul of the ladder (100 ng/ul) was loaded into the gel,…

A: Lane 1 bottom band has below 500 basepairs. Lane 1 top band has 5000bp. Lane 2 band 1000bp.

Q: Transcribed Image Text:Complete the following tasks. You discovered that a species of bacteria can…

A: Introduction  Gel electrophoresis is a laboratory technique for separating DNA, RNA, and protein…

Q: Compute for the appropriate amount (mL) of the compostions below of TE buffer used for DNA…

A: The appropriate amount of TE buffer needed for DNA extraction depends on the specific protocol or…

Q: List all the components and explain their purpose in the reaction mixture used for the dideoxy DNA…

A: The method of evaluating the sequence of nucleotides (As, Ts, Cs, and Gs) in a portion of DNA is DNA…

Q: Approximately how large is the Escherichia coli genome in basepairs? How many genes does it contain?

A: Escherichia coli also known as E. coli is a gram negative, facultative anaerobic, rod shaped,…

Q: What are the limitations of BLAST (Basic Local Alignment Search Tool)?

A: Introduction Basic local alignment search tool is an algorithm and programme for comparing primary…

Q: When y

A: Introduction: Deoxyribonucleic Acid, or DNA, is a molecule that transports genetic data in all…

Q: The algorithm or tool in MEGA that is used for multiple sequence alignment of nucleotide/DNA…

A: Introduction In bioinformatics MSA (Multiple Sequence Alignment)  is generally the alignment method…

Q: The following shows the results of DNA profiling from a rape case. The evidence sample (S) consists…

A: DNA profiling is the process in which a DNA pattern obtained from a person or sample, and this DNA…

Q: Aside from the sample and assuming proper AGE protocol, which of the following is most needed to…

A: Agarose gel  electrophoresis is an important technique used in molecular biology research. In case…

Q: Write precise and accurate differential report on the sequencing techniques

A: Genomics is the study of an organism's entire genome, which contains genetic elements. To sequence,…

Q: Suggest and describe two methods to detect DNA concentration by using in text citation and provide…

A: DNA is genetic material in almost all living organism that transit from one generation to another

Q: alphabetical index of your manuals. Why is this such an important rule to follow?

A: ICD-10-CM is International Classification of diseases, Tenth revision, and CM stands for clinical…

Q: In a PCR-based crime scene investigation, similar to the one presented in the lab module with…

A: The RAPD or rapid amplification of polymorphic DNA is used to detect the DNA that is present in the…

Q: Restriction Enzymes with LongerRecognition Sites Produce Larger _______ Fragments

A: The restriction enzyme is the molecular scissors that cut at a specific site. These are naturally…

Q: What is the sequence of the sample DNA submitted for sequencing given the gel electrophoresis…

A: Sanger sequencing method is chain terminating method.

Q: Describe in depth the steps that HMMs use to process vectors of continuous features.

A: Hidden Markov Models (HMMs) are a type of probabilistic model that can be used to model a wide range…

Q: What are the general lessons can you learn from QTL analyses?

A: Quantitative trait locus (QTL) analysis is a statistical tool for attempting to understand the…

Q: Probabilistic genotyping software allows for unbiased interpretation of a DNA profile using a(n)…

A: Probabilistic genotyping software allows for unbiased interpretation of a DNA profile using…

Q: Which of the following are necessary for Sanger sequencing? Select all correct answers. Group of…

A: Sanger sequencing method  was developed by the Frederick Sanger and his colleagues in 1977.This…

Q: Complete this Master Mix table for 8 DNA samples, a positive control, negative control, and an extra…

A: Volume per tube (uL) number of reactions volume in master mix (uL) PCR buffer 1.2 11 13.2 dNTP…

Q: Query Sequence A 0 Database Sequence B 10 0 5 Match 1 30 35 38 25 40 30 33 Match 2 Mismatch Match 50

A: Sequence alignment refers to the arrangement of the DNA/RNA/protein sequences to find out the…

Q: CPT code assignment 44970

A: Cpt code is current procedural terminology. These are the numerical codes helps to identify the…

Q: Evaluate the gel provided below. Use the notes to help you read the gel: DNA is loaded in the wells…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Inheritance and Mendelian Genetics

Inheritance means the passing of traits to offspring from parents. These traits could be passed either through asexual reproduction or sexual reproduction. The offspring receives the genetic material from the parents. Mendelian inheritance is a certain biological inheritance that follows the rules and principles laid down by Gregor Mendel in 1865. The initial hypothesis was considered wrong, but other inheritance theories modified the concept of inheritance and thus genetics.