Consider the following reaction catalyzed by Phosphopentose Epimerase at 37 °C. Xylulose 5-phosphate ---> Ribulose 5-phosphate At equilibrium, the ratio of Xylulose 5-P/Ribulose 5-P = 1/18.85 Calculate AGº Type your answer...

Consider the following reaction catalyzed by Phosphopentose Epimerase at 37 oC.                 Xylulose 5-phosphate      --->     Ribulose 5-phosphate At equilibrium, the ratio of Xylulose 5-P / Ribulose 5-P = 1/18.85 Calculate K’eq.

Consider the following reaction catalyzed by Phosphopentose Epimerase at 37 oC.                    Xylulose 5-phosphate      --->     Ribulose 5-phosphate At equilibrium, the ratio of Xylulose 5-P / Ribulose 5-P = 1/18.85  Which direction would the reaction proceed as written under standard conditions?

If 0.1 M glucose-1-phosphate is incubated with phosphoglucomutase, the glucose-1-phosphate is transformed to an isomer. At equilibrium, the concentration of glucose- 1-phosphate is 4.5 x 10'M and that of the isomer is 8.6 x 10 M. Calculate K' and AGo" for this reaction (i.e., in the direction of product formation). (R = 8.314 J/mol K)

Q2 CH,OH CH,OPO,2- H H OH H OH 0-PO,2- но но OH H. OH OH Glucose-1-phosphate Glucose-6-phosphate Calculate the standard free-energy change (AG°) fo the reaction catalyzed by the enzyme phosphoglucomutase. When at standard temperature (25 °C) and pressure, the equilibrium of each molecule is 1 mM glucose 1-phosphate and 19 mM glucose 6-phosphate. HINT: at 25 °C, RT = 2.479 kJ/mol

6. What class do the enzymes that catalyze the following reaction belong? (oxidoreductases, transferases, hydrolases, lyases, isomerases, ligases) H H H. C=C H-----H Hi H. H H ↑

C6H12O6 + a*O2 + b*NH3 --> c*CH1.64O0.52N0.16 + d*CO2 + e*H2O + f*C2H5OH In the aerobic yeast growth process, overflow metabolism can be observed when the sugar feed rate exceeds the oxidizing capacity of the yeast. In this case, part of the substrate is treated anaerobically and the production of biomass is reduced and ethanol is formed. This can also be observed when the respiratory rate RQ goes above one. The chemical composition of this yeast is CH1.64O0.52N0.16 . The yield of ethanol from glucose is 0.14 g g-1 and the yield of biomass from glucose is 0.362 g g-1. Calculate the oxygen demand of the reaction. In this calculation, use the following rounded even values as atomic weights for all calculations:  C=12 g/mol, H=1 g/mol, O=16 g/mol ja N=14 g/mol.  So how many moles of oxygen are needed per mole of glucose?

2. Experimental Determination of Keq' and AG If a 0.1 M solution of glucose 1-phosphate at 25 °C is incubated with a catalytic amount of phosphoglucomutase, the glucose 1-phosphate is transformed to glucose 6-phosphate. At equilibrium, the concentrations of the reaction components are Glucose1-phosphate glucose6-phosphate 9.6 x 10-2 M 4.5 x 10-3 M Calculate Keg' and AG for this reaction.

3. Hexokinase catalyzes the first reaction in glycolysis and phosphorylates D-glucose to D-glucose 6-phosphate using ATP as the source of the phosphate. Under conditions of pH 7, 25°C and a Hexokinase concentration of 3.0 nmol/mL, the KM for Hexokinase for the substrate glucose was determined to be 3.0 x 10 M. a. When the glucose concentration was set to 160 μM, the initial rate of the reaction was found to be 65.0 μmol/(mL s). What is Vmax for Hexokinase under these conditions? b. What is the turnover number for Hexokinase under these conditions? c. What is the catalytic efficiency for Hexokinase under these conditions?

The second step in the catabolism of most amino acids is the removal of the nitrogen atom from glutamate by oxidative deamination. In this reaction, glutamate is converted to an a-keto acid. Modify the structure to show the a-keto acid product. ?- || с I CH₂ I H₂N- & + remaining products enzyme CH₂ I CH₂ + NAD+ + H₂O CH₂ Incorrect Enter the chemical formulas for the remaining products. remaining products: NADH + H+ + NH Identify the enzyme that catalyzes the reaction. The enzyme that catalyzes the reaction is glutamate dehydrogenase. OH OH