Complete the data missing from the table, which shows assay data from the purification protocol of a hypothetical protein. Assay data Crude extract Total protein (mg) Total activity (units) Specific activity (units mg) Purification level Yield (%) 20,000 4,000,000 1 100 (NH4)2SO4 precipitation 5000 3,000,000 Affinity DEAE-cellulose Gel-filtration chromatography chromatography chromatography 500 45 1500 1,000,000 750,000 675,000
THIS IS ONE QUESTION/PROBLEM #18
FILL IN THE CHART // FILL IN THE EMPTY BOXES: I've written down what each empty box shows for the answer choices and labeled them as "options" (a, b, c, d). Each has its own box.
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Crude Extract --> Specific activity (units mg^-1) // options: (a) 5.0 x 10^-3 // (b) 200
(NH4)2SO4 precipitation --> Specific activity (units mg^-1) // options: (a) 600 // (b) 1.7 x 10^-3 // (c) 150
(NH4)2SO4 precipitation --> Purification level // options: (a) 3 // (b) 2 // (c) 0.34
(NH4)2SO4 precipitation --> Yield (%) // options: (a) 25 // (b) 75 // (c) 33
DEAE-cellulose chromatography --> Specific activity (units mg^-1) // options: (a) 1.5 x 10^-3 // (b) 667 // (c) 50
DEAE-cellulose chromatography --> Purification level // options: (a) 3.3 // (b) 0.30 // (c) 3 // (d) 1.1
DEAE-cellulose chromatography --> Yield(%) // options: (a) 33 // (b) 25 // (c) 30 // (d) 7.5
Gel-filtration chromatography --> Specific activity (units mg^-1) // options: (a) 1500 // (b) 37.5 // (c) 6.7 x 10^-4
Gel-filtration chromatography --> Purification level // options: (a) 4 // (b) 7.5 // (c) 0.13 // (d) 2.25
Gel-filtration chromatography --> Yield(%) // options: (a) 13 // (b) 75 // (c) 2.5 // (d) 19
Affinity chromatography --> Specific activity (units mg^-1) // options: (a) 6.7 x 10^-5 // (b) 15,000 // (c) 33.8
Affinity chromatography --> Purification level // options: (a) 5 // (b) 75 // (c) 0.01 // (d) 10
Affinity chromatography --> Yield(%) // options: (a) 0.2 // (b) 90 // (c) 1.3 // (d) 17
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