Q: Experiment :method of sterilization on glassware and media
A: Sterilization is a process by which all forms of life are killed, deactivated or removed from a…
Q: ame of Organism: Staphylococcus saprophyticus Type of Medium Blood Agar Color Before Inoculation…
A: Beta haemolysis represents an entire breakdown of the haemoglobin of the red blood cells within the…
Q: Why are the results of plate counts expressed as the number of colony-forming units, rather than the…
A: Microbes are small living organisms which can not be seen through naked eyes. The study of these…
Q: Difference between streak plate method and serial dilution
A: Ans: Streak plate method- It is a technique used mainly to isolate pure culture of bacteria from…
Q: You will only isolate pure colony to inoculate again (sub-culturing) to newly prepared plate medium,…
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: Citrate test What critical enzyme allows the organism to acquire carbon from the medium?
A: Citrate or citrate utilization test is one of the biochemical tests (IMViC tests) that are used for…
Q: Match the test with a positive result a. DNA test b. Paper chromatography test c. Celllar…
A: Genetic material is nothing but the sequence of nucleic acids which is called as DNA. It contains…
Q: Serial dilution is recommended prior to pour and spread plate. Why is this so?
A: Pour Plate: it is a technique in which A plate made by combining the inoculum which is cooled but…
Q: Inoculation Positive Results Negative Purpose Results Test Medium Reagents Mannitol Salts Loop Agar…
A: Biochemical reactions aid in identification of different bacterial species based on their…
Q: A. niger and B. cereus cultures used in this experiment containedspores. Why is B. cereus more heat…
A: Both Aspergillus niger and Bacillus cereus have spores of which the spores of B.cereus are more…
Q: amylasw test test for? medium+ - reagents + Test/why?
A: Amylase is an enzyme produced by the salivary glands to digest carbohydrates. The amylase test is…
Q: What is the purpose of the quadrant streak plate method? - How could this method be used in a…
A: In quadrant streak plate method, first of all the plate on which bacteria has to be grown that is on…
Q: How do you sterilize the following? petri dish inoculating loop culture media test tube…
A: Sterilization of following- petri dish - By using autoclave or by a series of steps that includes…
Q: Colonies of Bacillus cereus on (A) agar plate
A: Bacillus cereus it is gram positive, rod shaped , facultative anaerobic, spore forming, motile and…
Q: neration time of the species is 1 hour, and the microbes are in the exponential (log) hase of…
A: Microorganism that experience exponential growth its number increases according to this equation…
Q: PREPARATION OF SMEARS: 1. Wash the slide thoroughly with a detergent, rinse with water and dry with…
A: PREPARATION OF SMEAR ( FLOWCHART)
Q: Procedure Take ml Top up to 50ml. Add 50 ul of Mix Sml overnight culture mixture analyse to Add…
A: The graph emphasizes the different absorbance patterns of culture of E.coli and LB borth with…
Q: Observation 1. potato extract + phenol + H,02 2. potato extract + catechol + + H,0, 3. potato…
A: Catalase is an enzyme that acts as a biocatalyst. It is widely available in the peroxisomes of plant…
Q: Ninhydrin Test Samples used: Egg Albumin Gelatin Dispersion Added reagent: Ninhydrin Solution…
A: Ninhydrin is a general test for proteins and amino acids. It degrades amino acids into aldehydes,…
Q: Modified Acid-Fast Staining Procedure This technique is useful for the identification of the…
A: In laboratories, various techniques and staining procedures are used to determine the…
Q: purpose of using serum in culture media
A: A culture media is a media made in the lab, that is curated to facilitate the culture and growth of…
Q: Match the confirmation test to the category of bacteria. (each category can be used multiple times)…
A: Gram-positive bacteria are bacteria that test positive in the Gram stain test, which has…
Q: explain indole test, startch test and citrate test.
A: Indole test This is a biochemical test that is used to identify the capability of some bacteria to…
Q: What test can help you differentiate staphylococci from streptococci? What reagent or media would be…
A: Both are gram positive bacteria.
Q: Agar was not completely set prior to inoculating with the organism. Effect: Rationale:
A: Bacterial cultivation requires specific types of medium that can provide some of the basic…
Q: Bacterial Growth lab: Each 20-minute interval, absorbance of culture was recorded. When the curve…
A: Bacterial growth is defined as the proliferation of bacterial cells and increasing the number of…
Q: With liquid media such as broth, is it possible to determine if the culture is pure using the naked…
A: A culture is a method by which microbes are allowed to reproduce and multiply in predetermined…
Q: Stain Test. On a piece of white paper, place one or two drops of coconut oil. Note any stained…
A: Oils are liquids at room temperature due to the presence of higher percentage of unsaturated fatty…
Q: Colony formation can be observed in liquid media. a) Trueb) False
A: The culture medium or growth medium is the specific medium, which contains all essential nutrients,…
Q: The plates below were each created with 0.5 mL of culture from a 10-fold dilution series. Each plate…
A: Serial dilutions are utilised for the calculation of the concentration of microorganisms. Since it…
Q: Gram negative bacteria do not retain the stain when washed with alcohol.Comment.
A: Gram staining is a most important technique in the microbiology to differentiate the group of…
Q: discuss why a mixed culture cannot be used to inoculate a differential media such as the tripple…
A: A mixed culture is one that contains more than one type of organism growing in a sterile medium. The…
Q: Solve the identity of an unknown bacterial specimen by creating a dichotomous key. Categories:…
A: Microbes, which are tiny and nearly invisible, have had a huge influence on society since the…
Q: serology (elisa) lab: Why did you need to wash the wells after every step?
A: ELISA is the enzyme linked immunosorbent assay. The main components of ELISA are the antigens and…
Q: Procedure Add 50 of ul the Mix Sml Take 3 ml Top up to 50ml. overnight culture with 45 ml LB -…
A:
Q: Citrate test what is the color of the medium after incubation? Green or blue
A: Citrate or citrate utilization test is one of the biochemical tests (IMViC tests) that are used for…
Q: Create a flow chart to perform the required tests to identify unknown microorganism: - Use at least…
A: Introduction :- Micro-organisms are classified into different categories on the basis of the…
Q: Give 2 different reasons that scientists might need to isolate a pure culture.
A: Pure culture: a culture of microorganism (usually bacteria) that contain only single type of…
Q: Derailed description of the following test / media SIM medium
A: SIM stands for Sulfide, Iodide, Motility medium. It is a semi-solid medium that is used to check the…
Q: which kind of sample gives positive result for Molish test?
A: It is a chemical test which is named after the botanist Hans Molisch. This test involves the…
Q: DESCRIBE THE BACTERIA OBSERVED. DO NOT DEFINE THE STAINING TECHNIQUES. PLEASE REFER TO THE PHOTO.
A: Microbes, which are tiny and nearly invisible, have had a huge influence on society since the…
Q: Explain why some cells are pink and others are the Gram- stained bacterial smear.
A: Gram staining is the differential staining technique performed to differentiate the bacterial…
Q: Subculture and Colony Morphology Descriptions Following identification of the Gram-negative isolate…
A: Microbiology is the field of biology that deals with the study of organisms, which cannot be seen…
Q: Citrate test What pH idicateor is in this medium?
A: It is required to identify the pH indicator in citrate test.
Q: Catalase test What is the reagent used in this test?
A: It is required to identify the reagents that are used in the Catalase test.
Q: What is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tube
A: Bacterial culture media is a kind of growth media in which the required bacteria is cultured. The…
Q: Kligler’s iron agar and SIM are multiple test media.. Both media are stabbed but for different…
A: The liquid or solid substratum on which, the organ or cell explants can be grown. The medium…
Q: In product testing, no colonies appeared. Interpretation: Rationale:
A: Ans: Product testing: The product testing is performed as a regular quality control protocol in…
Citrate test
What is the color of the medium before inoculation? Green or blue
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
- What is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide 2. To cause the cells to shrink and become distorted 3. To kill the bacteria so they aren't harmed by the staining method 4. To break down the cell wall in order to make the cells accept stain 5. To kill the bacteria to make the slide safer to handleDESCRIBE THE BACTERIA OBSERVED. DO NOT DEFINE THE STAINING TECHNIQUES. PLEASE REFER TO THE PHOTO.Why the microorganisms in the hay infusion are not stained?
- This is how a hanging-drop slide is prepared. Figure from Macedo, Wikimedia Commons, 2016. 2 3 4 slide cover slip vaseline concavity drop of microbiological culture CA inoculation loop 11. Which slide gives you more information, the hanging drop or the stained slide? Why do you say so? 12. Why might it be valuable to know whether a bacterium is motile?Why is a block of agar inoculated rather than streaking the fungal culture on a plate?If you prepare culture media plates 2 days ahead of the laboratory schedule, can you still use the pre-made plates by the time of the laboratory schedule?