The hydrolysis of carbobenzoxyglycyle-L- tryptophan catalyzed by the pancreatic carboxypeptidase was carried out according to the following equation. Carbobenzoxyglycyle - L- tryptophan + H,0 → carbobenzoxyglycyine +L- tryptophan The data below represent the rate of formation of L – tryptophan at pH = 7.5 ,25°C. The results are listed as follows [S] (mM) Rate(mMs 2.5 5.0 10.0 15.0 20.0 0.024 0.053 0.060 0.064 Yusra Plot these data according to Line-Weaver - Burke equation, then find the values of vmar and km?
Catalysis and Enzymatic Reactions
Catalysis is the kind of chemical reaction in which the rate (speed) of a reaction is enhanced by the catalyst which is not consumed during the process of reaction and afterward it is removed when the catalyst is not used to make up the impurity in the product. The enzymatic reaction is the reaction that is catalyzed via enzymes.
Lock And Key Model
The lock-and-key model is used to describe the catalytic enzyme activity, based on the interaction between enzyme and substrate. This model considers the lock as an enzyme and the key as a substrate to explain this model. The concept of how a unique distinct key only can have the access to open a particular lock resembles how the specific substrate can only fit into the particular active site of the enzyme. This is significant in understanding the intermolecular interaction between proteins and plays a vital role in drug interaction.
![The hydrolysis of carbobenzoxyglycyle-L- tryptophan catalyzed by the
pancreatic carboxypeptidase was carried out according to the following
equation.
Carbobenzoxyglycyle - L- tryptophan + H20
→ carbobenzoxyglycyine + L- tryptoj
The data below represent the rate of formation of L- tryptophan at
pH = 7.5 ,25°C. The results are listed as follows:
[S] (mM)
2.5
10.0
5.0
15.0
20.0
Rate(mMs
0.024
0.053
0.060
0.064
Yusra
Plot these data according to Line-Weaver - Burke equation, then find the
values of vmar and km?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Ff3c93712-c3ae-4460-8a96-9b94ed644ca1%2F42ad07ce-bade-459a-acb9-55b0be701c3e%2Fvi4tq7_processed.jpeg&w=3840&q=75)
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