Answered: Can you use Sephadex G-75 to separate…

Can you use Sephadex G-75 to separate alcohol dehydrogenase (MW 150000 grams/moles) from beta-amylose (MW of 120000 grams/moles) using gel filtration chromotography? Why or why not?

Introduction to General, Organic and Biochemistry

11th Edition

ISBN:9781285869759

Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Chapter30: Nutrition

Section: Chapter Questions

Problem 30.3P: If sodium benzoate, a food preservative, is excreted as such and if calcium propionate, another food...

See similar textbooks

Related questions

Q: None

A: Given Data:Total mass of red gold (M): 8.68 gTotal volume of red gold (V): 0.514 cm³Density of gold…

Q: Give detailed mechanism Solution with explanation needed. don't give Ai generated solution

A: Acid-base neutralization reaction according to Bronsted-Lowry concept:According to this concept, an…

Q: For the element lead Pb please answer the questions and be specific: 1. Write its proper…

A: Step 1:Answer1. The chemical symbol for lead is Pb.Isotope: Isotopes are distinct nuclear species…

Q: P+ HELP CENTER כC) I\CSVianICCTVT D) Stereoisomers Question 10 (Mandatory) What is the relationship…

A: Step 1: IsomersEnantiomers: Those stereoisomers that possess non-superimposable mirror images of…

Q: Propose a mechanism for the following reaction. CH2CH2CH2CH2 + H₂O OH OH

A: The given reaction is the acid-catalyzed reaction for cyclic ether synthesis.ExplanationIn acid…

Q: Please correct answer and don't use hand rating

A: Step 1:Find the average atomic mass of element J: To find the average atomic mass of element J, we…

Q: Please predict the products for each of the following reactions. Clearly show the regiochemistry…

Q: The following diagram represents a reaction reaching equilibrium. Each molecule in the diagram…

A: Let the B is blue colored ball and A is red colored ball.Then the above reaction will follows the…

Q: s of Organic Chem x OWLV2 | Online teaching and le × +…

A: Thank you.

Q: Do all atoms of the sane element contain the same number of protons? What about neutrons?

A: Atoms are the basic units of matter and the defining structure of elements. The term 'atom' comes…

Q: 1. (6 points) Draw clear pictures showing the two chair conformations of this compound. Circle the…

Q: If Kc = 82 for the reaction below at 200.0 K, then what is the value of Kp? (R = 0.0821…

A: The relationship between Kp and Kc is given by the equation Kp = Kc(RT)ᵟn, where R is the ideal gas…

Q: Calculate strain energy for the conformer pictured below, using strain energy increments from the…

Q: Calculate strain energy for the conformer picture below, using the strain energy increments from the…

Q: Look over the flow chart you see below and figure out whichcompound you expect to identify at each…

Q: None

Q: On the molecule below, highlight primary halide carbons in red, secondary halide carbons in blue,…

A: Thank you.

Q: Steps and explanation please.

A: Approach to solving the question: acid base reaction, thermodynamics Detailed explanation: Key…

Q: Determine whether the following molecule is a hemiacetal, acetal, or neither and select the…

A: Step 1:I see, let's revisit this carefully.In the molecule you provided, we need to identify whether…

Q: Practice help with both not a graded ass

A: Problem 5:Problem 6: The study of molecules through the recording of radiofrequency electromagnetic…

Q: A particular refrigerator cools by evaporating liquefied dichlorodifluoromethane, CCl2F2. How many…

A: First, we need to calculate the number of moles of water. The molar mass of water is approximately…

Q: None

Q: Question 8

A: Step 1: To do this, we must compute the moles of each solution using the molarity and volume, and…

Q: j er of 's for each of the formulas in (1.). 3. For each M*: M*+2 pair in (1.) give the ratio of M*…

A: Approach to solving the question: mass spectroscopy Detailed explanation: Key references:

Q: For the reaction: X(g) + Y-----> XY(g) The rate law is given by the expression, Rate= k [Y]3 .…

A: The rate law of a chemical reaction is a mathematical relationship obtained by comparing reaction…

Q: Identify the atoms which have the following electron configurations: 1s22s22p63s23p64s13d2:…

A: Electron configuration is the distribution of electrons of an atom or molecule in atomic or…

Q: Please correct answer and don't use hand rating

A: Step 1:Step 2: Step 3: Step 4:

Q: Why is the Fe-Fe3-C phase diagram a metastable phase diagram instead of a true equilibrium phase…

A: The Fe-Fe3-C phase diagram, also known as the iron-carbon phase diagram, is a graphical…

Q: 15. The compounds below are the conjugate bases of the acids listed on the right. Using the pKa…

A: From the pKa value how strong the acid is can be predicted. Higher is the pKa value, weaker is the…

Q: 0.219 g of salicylic acid and 0.676 mL of acetic anhydride (d = 1.082 g/mL) react in the presence of…

A: The first step in this process is to determine the molar mass of the reactants and the product. The…

Q: Hi can you help me find the chemical reaction and molecular structure of this Nmr.

A: which is a visual representation of the separation of components in a mixture. Here's a breakdown of…

Q: In the compound below, identify all carbon atoms that are electron deficient (8+) and all carbon…

A: Thank you.

Q: The rate constant for the reaction in question (1) is 7.2x109 at 298 K. Using your answer from…

A: Step 1- To find the rate constant at 205 K,use the Arrhenius equation: k = Ae^(-Ea/RT) where:k =…

Q: If 0.128 g of hydrogen plus ions is dissolved in 250.0mL of water, calculate the concentration of H+…

A: Approach to solving the question: Detailed explanation:Step 1: Convert first the mass of H+ to mole…

Q: Please correct answer and don't use hand rating

Q: 3.11 Suppose that when you exercise, you consume 100 g of glucose and that all the energy released…

Q: None

A: The electron pair acceptor is labeled as a Lewis acid, while the electron pair donor is labeled as a…

Q: a) Write the relative reaction rate equations for the generic reaction:A + 2B → C b) In the lab,…

A: The rate of a chemical reaction is generally expressed as the change in concentration of reactants…

Q: Given the following reaction and the equilibrium concentrations of .70M for H2S and .30M for HI,…

A: Let's use a general equation to write the equilibrium constant expression:aA + bB ←→ cC +…

Q: Classify each of the molecules below. O X CH3-CH2-C-NH2 O 1° amide O 2° amide O 3° amide CH3-C-NH2 O…

A: Thank you.

Q: Please correct answer and don't use hand rating

A: The images you provided correctly depict the sigma bonding (σ) and antibonding (σ*) orbitals for the…

Q: For the information given below predict the structure of the unknown compound using the information…

A: Step 1: Step 2: Step 3: Step 4:

Q: Hi I know that the red is the correct one and it's like the nmr but Chan you explain why. Also in…

A: The image you provided shows the NMR spectra of two compounds, labeled "1en" and "1ex." Based on the…

Q: 6. Calculate the solubility of Ni(OH)2 when buffered to pH 9.0. (Ksp of Ni(OH)2 is 6.0 X 10-16)

A: The problem is asking us to calculate the solubility of nickel(II) hydroxide, Ni(OH)2, in a solution…

Q: 4. One resonance form of a cation is shown below on the left. Complete its two resonance structures…

A: Answer: For resonance structure:The delocalized electrons move to form the resonance structures.…

Q: Define the following phases that exist in the Fe-Fe3-C phase diagram: (iv) ferrite.

A: The Fe-Fe3-C phase diagram, also known as the iron-carbon phase diagram, describes the phase…

Q: Complete the table by providing each combination equation and provide Solid (S) or No reaction (NR)…

A: Step 1:…

Q: Can you explain how you find the answer? And the steps you took? I believe the answer is C but I am…

A: We apply the Cahn-Ingold-Prelog priority rules to identify if a molecule has a "R" or "S"…

Q: Arrange the following groups in the order of increasing priority according to the Cahn-Ingold-Prelog…

Q: find the stretching frequenc

A: Infrared (IR) spectroscopy is a powerful tool in organic chemistry that allows us to identify the…

Question

Can you use Sephadex G-75 to separate alcohol dehydrogenase (MW 150000 grams/moles) from beta-amylose (MW of 120000 grams/moles) using gel filtration chromotography? Why or why not?

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Similar questions

If sodium benzoate, a food preservative, is excreted as such and if calcium propionate, another food preservative, is metabolized to CO2 and H2O, would you consider either of these preservatives to be a nutrient? If so, why?
An enzymatic assay was performed using the enzyme phophofructokinase-1, which catalyses the committed step of the glycolysis pathway. What is the final concentration of the fructose 6-phosphate substrate in a test tube containing the following: 250 µL of 150pM fructose 6-phophate, 100 µL 20 mg.ml-1 phosphofructoskinase-1 and 150 µL 100mM buffer pH 7.5
Please answer fast i give you upvote.
OF as fil PDF What are the products of the reaction of the triacylglycerol found in coconut oil (containing only lauric acid) with KOH? O UNOFFIC TRANSCR 9°F Mostly cloudy CH,−O–C−(CH2)10—CH3 0 CH-0-C-(CH₂)10-CH3 + 3KOH O CH,−O−C(CH2)10—CH3 Glyceryl trilaurate O one glycerol molecule and three potassium laurate formula units O one glycerol molecule and three sodium laurate formula units O one glycerol molecule and three lauric acid molecules O one glycerol molecule and three potassium myristate formula units A Heat, 10 O Search ac PD PD maste omiss pe huniz recor C
1
EQD₂ = nd* α Assume a/B (d+(a/B)) (2+(a/B)) BED:= (nd)*(1+ α =2 for late effects and a/B 10 for acute effects 1. Using the Equivalent Dose in 2 Gy Fractions equation, compare the following protocols. Which would have better tumor control? Which would have worse late effects? 42 Gy delivered in 12 fractions; 30 Gy delivered in 5 fractions.
I need help with my homework
Please don't provide handwriting solution
You have learned quite a number of different physicochemical methods for fractionating the contents of cellular extracts and the proteins contained within them. Which technique would be the "best" to perform the following task. Removing K+ ions from a protein sample . Oion exchange salting out differential centrifugation O gel filtration O dialysis
Name two more methods, besides the Bradford Assay, for determining protein concentration.
Hey, I was wondering if someone could check my work and explain to me what I'm doing wrong if otherwise. Thanks!
Bha Please don't provide the handwriting solution