Calculate the maximum velocity (Vmax) of the mutant glucokinase to one decimal place using the Michaelis-Menten equation.

Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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A researcher purifies a mutant version of the glucokinase enzyme. She combines the purified glucokinase with glucose and ATP to
produce glucose 6-phosphate. She records the initial velocity (Vo) of glucose 6-phosphate production at various substrate
concentrations ([S]). She determines a Michaelis-Menten constant (Km) of 6.2 mM for the mutant enzyme.
[S] (mm)
5
10
50
100
Vas
Vo
umal
min
1.03
1.42
2.05
2.17
Calculate the maximum velocity (Vmax) of the mutant glucokinase to one decimal place using the Michaelis-Menten equation.
umol
min
Transcribed Image Text:A researcher purifies a mutant version of the glucokinase enzyme. She combines the purified glucokinase with glucose and ATP to produce glucose 6-phosphate. She records the initial velocity (Vo) of glucose 6-phosphate production at various substrate concentrations ([S]). She determines a Michaelis-Menten constant (Km) of 6.2 mM for the mutant enzyme. [S] (mm) 5 10 50 100 Vas Vo umal min 1.03 1.42 2.05 2.17 Calculate the maximum velocity (Vmax) of the mutant glucokinase to one decimal place using the Michaelis-Menten equation. umol min
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