Calculate the concentration of viable cells per mL In your resuspended sample giving your answer to 3 decimal places [ Number x 108 cells/mL. You receive a second cell sample from another researcher to include in your experiment. These cells are resuspended at a concentration of 2 x 10 cells/mL. For each sample you run on the flow cytometer you require 250,000 cells. Calculate the volume of the cell suspension that you need to add to each FACS tube. Give your answer in microlitres (L) to 1 decimal place,
Calculate the concentration of viable cells per mL In your resuspended sample giving your answer to 3 decimal places [ Number x 108 cells/mL. You receive a second cell sample from another researcher to include in your experiment. These cells are resuspended at a concentration of 2 x 10 cells/mL. For each sample you run on the flow cytometer you require 250,000 cells. Calculate the volume of the cell suspension that you need to add to each FACS tube. Give your answer in microlitres (L) to 1 decimal place,
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question
![/Coursework 2
Remaining Time: 04:30:04
You have cultured some cells for a flow cytometry experiment. The cell concentration has been
calculated using an automated cell counter as 1.5 x 10° cells/mL, and the overall viability of the cells is
90%. You collect your cells, wash them and resuspend in a final volume of 1.5 mL of FACS buffer.
Calculate the concentration of viable cells per mL in your resuspended sample giving your
answer to 3 decimal places [
Number
x 108 cells/mL.
You receive a second cell sample from another researcher to include in your experiment. These cells are
resuspended at a concentration of 2 x 10 cells/mL. For each sample you run on the flow cytometer you
require 250,000 cells.
Calculate the volume of the cell suspension that you need to add to each FACS tube. Give your
answer in microlitres (L) to 1 decimal place,
Number
AL
You have made the flow cytometry sample up to a final volume of 250μL and now need to label your
sample with an antibody. The antibody stock solution is 0.5mg/mL and needs to be diluted to a final
working concentration of 6.2 μg/mL.
What volume of stock antibody solution do you need to add to your sample tube to achieve the
required working concentration? Give your answer in μL to 1 decimal pla
Number
115
HL
MAR
30
10₂
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Transcribed Image Text:/Coursework 2
Remaining Time: 04:30:04
You have cultured some cells for a flow cytometry experiment. The cell concentration has been
calculated using an automated cell counter as 1.5 x 10° cells/mL, and the overall viability of the cells is
90%. You collect your cells, wash them and resuspend in a final volume of 1.5 mL of FACS buffer.
Calculate the concentration of viable cells per mL in your resuspended sample giving your
answer to 3 decimal places [
Number
x 108 cells/mL.
You receive a second cell sample from another researcher to include in your experiment. These cells are
resuspended at a concentration of 2 x 10 cells/mL. For each sample you run on the flow cytometer you
require 250,000 cells.
Calculate the volume of the cell suspension that you need to add to each FACS tube. Give your
answer in microlitres (L) to 1 decimal place,
Number
AL
You have made the flow cytometry sample up to a final volume of 250μL and now need to label your
sample with an antibody. The antibody stock solution is 0.5mg/mL and needs to be diluted to a final
working concentration of 6.2 μg/mL.
What volume of stock antibody solution do you need to add to your sample tube to achieve the
required working concentration? Give your answer in μL to 1 decimal pla
Number
115
HL
MAR
30
10₂
MacBook Air
tv
F7
Submit Assignment
DII
F8
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