In a monolayer of cultured epithelial cells, the cells may or may not have formed tight junctions. What experimental test could be used to determine if the cells have functional tight junction structures? Place a small dye (<1 k Da in molecular weight) into the culture media of cells cultured on a glass coverslip and use live imaging to monitor whether the dye can penetrate into the O cytoplasm of the cells, as would be expected if the cells have functional tight junctions. Place a smalldye (<1 k Da in molecular weight) on the apical side of a cell monolayer cultured on a permeable membrane and use live imaging to detemine if the dye remained on the O apical side, as would be expected if the cells have functional tight junctions. Place a large dye (>1 k Da in molecular weight) into the culture media of cells cultured on a glass coverslip and use live imaging to monitor whether the dye can penetrate into the O cytoplasm of the cells, as would be expected if the cells have functional tight junctions. Inject a cell with a small dye (<1 kDa in molecular weight) and use live cell imaging to track whether the dye moves into neighboning cells, as would be expected if the cells have functional O tight junctions.
Cell Structure
The knowledge and concept about the structure of the cells have changed constantly over the past years. Primarily the scientist defines the cells as a simple membranous sac with fluids and some particles. Researches have now revealed that the cell is the more complex and basic unit of life. There are different types of cells and they also differ in shape and structure. The basic three parts of a cell are cell membrane, cytoplasm, and organelle.
Plant Cell
The smallest functional unit of a living organism is known as the cell. It is composed of cytoplasm enclosed by an outer layer known as the cell membrane. A cell is classified as a prokaryotic cell and a eukaryotic cell. A prokaryotic cell does not contain a nucleus, and the genetic material is freely suspended in the cell cytoplasm; for example, bacteria are composed of a prokaryotic cell. Whereas a eukaryotic cell contains a nucleus and membrane-bound cell organelles with a specific function, plants and animal cells are examples of eukaryotic cells.
Plant Cell Types
Plants are made up of a variety of cell types that include both living and dead cells. These cells provide them mechanical and structural support along with the growth of plants. Plant tissue is a group or collection of cells, which are similar in structure and work together to accomplish a specific function. Plant cells are most similar to animal cells, both being eukaryotic cells.
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
