Calculate the amount of each chemical required to make a 10x stock of TBE in a finalvolume of 150 mL (Table 7)Molecular weight and final concentration (mM) are given. The chemicals aredissolved in distilled waterTable 7 Preparing 10x TBEComponentTris BaseBoric AcidEDTA*MolecularWeightorConcentration121.14 g/mol1 M372.24 g/mol*Ethylenediaminetetraacetic acid10x StockAmount(grams or mL)needed for 150 mLStockConcentrationto make890 mM890 mM20 mMFinal 1xWorkingConcentrationto run gel89 mM89 mM2 mM

We know that, V×M = number of mmol Where V = volume & M = molarity of the solution.

● ● Calculate the amount of each chemical required to make a 10x stock of TBE in a final volume of 150 mL (Table 7) Molecular weight and final concentration (mM) are given. The chemicals are dissolved in distilled water Table 7 Preparing 10x TBE Component Tris Base Boric Acid Molecular Weight or Concentration 121.14 g/mol 1 M 10x Stock Amount (grams or mL) needed for 150 mL Stock Concentration to make 890 mM 890 mM Final 1x Working Concentration to run gel 89 mM 89 mM

● ● Calculate the amount of each chemical required to make a 10x stock of TBE in a final volume of 150 mL (Table 7) Molecular weight and final concentration (mM) are given. The chemicals are dissolved in distilled water Table 7 Preparing 10x TBE Component Tris Base Boric Acid Molecular Weight or Concentration 121.14 g/mol 1 M 10x Stock Amount (grams or mL) needed for 150 mL Stock Concentration to make 890 mM 890 mM Final 1x Working Concentration to run gel 89 mM 89 mM

Chemistry: Principles and Practice

3rd Edition

ISBN:9780534420123

Author:Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer

Publisher:Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer

Chapter16: Reactions Between Acids And Bases

Section: Chapter Questions

Problem 16.9QE

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