c. Using the pк data for lysine, draw the molecular species present at each of the following pH values and determine the overall charge on the population of molecules. pH 1.0 pH 2.2 pH 5.6 pH 9.5 pH 12

Polymer beads (resin) made of DEAE (diethylaminoethyl) cellulose are packed in an ion exchange column. The total mass of beads in the column is 8.47 kg. On average, each bead weighs 0.0023 g and has an average of 18.4 * 10° positively charged amine groups that can adsorba negatively charged protein that passes through the column. A solution containing 2.07 mg/L of a protein is maintained at pH 6.3 and is passed through the ion exchange column at 0.215 L/min. The protein has a molecular weight of 154,000. The pk, of the amino groups on DEAE cellulose is 7.1, and the pl of the protein is 5.6. 2. A. How long can the column be operated before reaching 80% capacity (i.e., 80% of the amino groups on DEAE are bound to the protein through an ionic bond)? You may assume that one protein attaches to one + charge on the beads (although it's possible that proteins attach to more than one + charge). B. After reaching 80% capacity, explain what you would do to release the protein attached to the…

Enter an estimate for the charge on the peptide KPIVRFDEESC at pH 8.0.

Remember that DNA is a sequence of the letters A,C,T, and G. A binds with T and C binds with G. Solutions are specified by the DNA sequence, such as ACAC or TG, and by the concentration in units of micromolar (uM = 10-M) Consider mixing 30ml of 0.30µM ACAC with 45ml of 0.50pM TG. Predict what reactants and products should be present in the final solution and then check your answer in the virtual lab. (Please give your final answer in total nanomoles (10 moles) for each species in the solution)

Calculate the salt to acid ratio ([Aˉ]/[HA]) of a solution of an amino acid at pH 2 and  pKa of 8.95

A protein gives, under conditions of buffer composition, pH, and temperature that are  close to physiological conditions, a molecular weight by sedimentation equilibrium  measurements of 140,000 g/mol. When the same protein is studied by SDS gel  electrophoresis in the absence or presence of the reducing agent β-mercaptoethanol  (BME), the patterns seen in lanes A and B respectively are observed. Lane C contains  standards of molecular weight indicated. From these data, describe the native protein,  in terms of the kinds of subunits present, the stoichiometry of subunits (that is, how  many of each subunits are present), and the kinds of bonding (covalent, non-covalent)  existing between subunits

A mixture of proteins contains four different polypeptides, all in ~equal concentration, in solution with the following properties: Protein Molecular Mass (kDa)        Isoelectric point A                 45                                        4.5 B                 77                                        6.0 C                 28                                        4.1 D                 14                                        10.7   A fraction of the protein solution is applied to a strong cation exchange column using a buffer at pH 8.0 with increasing [NaCl] from 0.05 M – 1.0 M.  The chromatogram is shown below: 1. Based on the data presented, which of the following statements is true: Peak #1 is protein D Peak #4 is protein C Peak #3 is protein A Peak #4 is protein D Peak #2 is protein B 2. Since you know that the proteins are all present in approximately equal concentrations, the different relative peak areas tell you that: There are more neutral amino acids in protein #4…

Given the figure below. Which structure is predominant at pH = 8.0? (Note: The amino acid structures on the topmost portion are labeled as A, B, C and D from left to right) * çOOH pK1 H3Nt CH CH2 CH2 CH2 ČH2 NH3* poo H2N-CH CH2 CH2 GH2 CH2 NH2 foo H2N-CH CH2 çoo pK2 pk3 CH2 CH2 NH3* NH3* 14 1 pK2 pl pk3 12 10 pH 6 4 pK1 2 0.5 1 1.5 2 2.5 3 Equivalents of OH

please draw anr label the 20 amino acids found in proteins as they would exist in an aqueous solution at pH=7.4

In the Bradford protein determination, the color of a dye changes from brown to blue when it binds to a protein.  The intensity of blue color (measured by absorbance of light at a wavelength of 595nm) is proportional to protein concentration. Protein (ug) Absorbance at 595nm 0.00 0.466 9.36 0.676 18.72 0.883 28.08 1.086 37.44 1.280   Use Excel to determine the equation of the line put it here in the format of y = mx + b.  Start your answer with "y=". Using the calibration curve for the Bradford protein determination in the previous question, determine the number of micrograms of protein in an unknown if it gave an absorbance of 0.973.  Report answer to the hundredths place.