Examine the structure of the pBR322 plasmid depicted below. Assume total size of the plasmid is 4,361 bp and the blue numbers indicate locations of restriction sites relative to the O point at the top of the plasmid. What size fragments would be generated by the following restriction digestion reactions? 1. Sall 2. Sal 1 + BamH1 3. Sal 1 + EcoR1 4. Sal I + BamH1 + EcoR1 PstI 3607 3000 4000 amp ori HindIII Edit View Insert Format Tools Table EcoRI EcoRV 4359 0 29 185 pBR322 4361 bp 2295 NdeI tet 2000 BamHI 375 651 SalI 1000 Type a short answer in the space provided below.

Human Anatomy & Physiology (11th Edition)
11th Edition
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Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Examine the structure of the pBR322 plasmid depicted below. Assume total size of the plasmid is
4,361 bp and the blue numbers indicate locations of restriction sites relative to the O point at the
top of the plasmid. What size fragments would be generated by the following restriction digestion
reactions?
1. Sall
2. Sal 1 + BamH1
3. Sal 1 + EcoR1
4. Sal I + BamH1 + EcoR1
PstI
3607
3000
4000
amp
ori
HindIII
Edit View Insert Format Tools Table
EcoRI EcoRV
4359 0 29 185
pBR322
4361 bp
2295
NdeI
tet
2000
BamHI
375
651
SalI
1000
Type a short answer in the space provided below.
Transcribed Image Text:Examine the structure of the pBR322 plasmid depicted below. Assume total size of the plasmid is 4,361 bp and the blue numbers indicate locations of restriction sites relative to the O point at the top of the plasmid. What size fragments would be generated by the following restriction digestion reactions? 1. Sall 2. Sal 1 + BamH1 3. Sal 1 + EcoR1 4. Sal I + BamH1 + EcoR1 PstI 3607 3000 4000 amp ori HindIII Edit View Insert Format Tools Table EcoRI EcoRV 4359 0 29 185 pBR322 4361 bp 2295 NdeI tet 2000 BamHI 375 651 SalI 1000 Type a short answer in the space provided below.
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