Table 4- Determination of the optimum pH of catechol oxidase enzyme Potato extract (mL) Tube # 2d 3d 4d 5d 6d 7d dH₂O (mL) 0 0 0 0 0 0 Catechol (mL) 6 6 6 6 6 6 pH buffer added 4mL pH 2 4mL pH 4 4mL pH 6 4mL pH 7 4mL pH 8 4mL pH 10 1 1 1 1 1 1 Absorbance (0 mins) A: 0.010 Start Time: 3:24 A: 0.008 Start Time: 3:34 A: 0.023 Start Time: 3:36 A: -0.006 Start Time: 3:36 0.018 A: Start Time: 3:36 A: 0.011 Start Time: 3:36 Absorbance (after 10 mins in 40°C water bath) -0.030 A: Take reading at: 3:44 A: 0.154 Take reading at: 3:46 A: 0-105 Take reading at: 3:46 A: 0-132 Take reading at: 3:46 A: 0.074 Take reading at: 3:46 A: 0.018 Take reading at: 3!46 Q15) What is the enzyme's optimum pH(s)? Answer: Q16) Use the difference between 2nd and 1st absorbance to support your conclusion for the optimum pH. Subtract Omin from 10min absorbance -0.04 0.0012 0.082 0.138 0.056 0.007
Table 4- Determination of the optimum pH of catechol oxidase enzyme Potato extract (mL) Tube # 2d 3d 4d 5d 6d 7d dH₂O (mL) 0 0 0 0 0 0 Catechol (mL) 6 6 6 6 6 6 pH buffer added 4mL pH 2 4mL pH 4 4mL pH 6 4mL pH 7 4mL pH 8 4mL pH 10 1 1 1 1 1 1 Absorbance (0 mins) A: 0.010 Start Time: 3:24 A: 0.008 Start Time: 3:34 A: 0.023 Start Time: 3:36 A: -0.006 Start Time: 3:36 0.018 A: Start Time: 3:36 A: 0.011 Start Time: 3:36 Absorbance (after 10 mins in 40°C water bath) -0.030 A: Take reading at: 3:44 A: 0.154 Take reading at: 3:46 A: 0-105 Take reading at: 3:46 A: 0-132 Take reading at: 3:46 A: 0.074 Take reading at: 3:46 A: 0.018 Take reading at: 3!46 Q15) What is the enzyme's optimum pH(s)? Answer: Q16) Use the difference between 2nd and 1st absorbance to support your conclusion for the optimum pH. Subtract Omin from 10min absorbance -0.04 0.0012 0.082 0.138 0.056 0.007
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Transcribed Image Text:Table 4- Determination of the optimum pH of catechol oxidase enzyme
Potato
extract
(mL)
Tube #
2d
3d
4d
5d
6d
7d
dH₂O
(mL)
0
0
0
0
0
0
Catechol
(mL)
6
6
6
6
6
6
pH buffer
added
4mL pH 2
4mL pH 4
4mL pH 6
4mL pH 7
4mL pH 8
4mL pH 10
1
1
1
1
1
1
Absorbance
(0 mins)
A: 0.010
Start Time: 3:24
A: 0.008
Start Time: 3:34
A: 0.023
Start Time: 3:36
A:
-0.006
Start Time: 3:36
0.018
A:
Start Time: 3:36
A:
0.011
Start Time: 3:36
Absorbance
(after 10 mins in 40°C
water bath)
-0.030
A:
Take reading at: 3:44
A: 0.154
Take reading at: 3:46
A: 0-105
Take reading at: 3:46
A:
0-132
Take reading at: 3:46
A: 0.074
Take reading at: 3:46
A: 0.018
Take reading at: 3!46
Q15) What is the enzyme's optimum pH(s)? Answer:
Q16) Use the difference between 2nd and 1st absorbance to support your conclusion for the optimum pH.
Subtract Omin
from 10min
absorbance
-0.04
0.0012
0.082
0.138
0.056
0.007
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