Be sure to answer all parts. A microbiologist is preparing a medium on which to culture E. coli bacteria. She buffers the medium at pH 7.00 to minimize the effect of acid-producing fermentation. What volumes of equimolar aqueous solutions of K2HPO, and KH,PO, must she combine to make 900.0 mL of the pH 7.00 buffer? K, values for phosphoric acid: Ka1 = 7.2 x 103 K22 = 6.3 x 10-8 Ka3 = 4.2 x 10-13 Volume H,PO, = | mL Volume HPO,: mL

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Chapter1: Chemical Foundations
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Be sure to answer all parts.
A microbiologist is preparing a medium on which to culture E. coli bacteria. She buffers the medium at
pH 7.00 to minimize the effect of acid-producing fermentation. What volumes of equimolar aqueous
solutions of K,HP04 and KH,PO4 must she combine to make 900.0 mL of the pH 7.00 buffer?
Ka values for phosphoric acid:
Kal = 7.2 x 10-3
Ka2 = 6.3 x 10-8
Ka3 = 4.2 x 10-13
Volume H,PO,:
mL
Volume HPO,
mL
!!
Transcribed Image Text:Be sure to answer all parts. A microbiologist is preparing a medium on which to culture E. coli bacteria. She buffers the medium at pH 7.00 to minimize the effect of acid-producing fermentation. What volumes of equimolar aqueous solutions of K,HP04 and KH,PO4 must she combine to make 900.0 mL of the pH 7.00 buffer? Ka values for phosphoric acid: Kal = 7.2 x 10-3 Ka2 = 6.3 x 10-8 Ka3 = 4.2 x 10-13 Volume H,PO,: mL Volume HPO, mL !!
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