helping tags: Biochemistry, spectrophotometric analysis, enzyme, substrate, coenzymeWill upvote, just pls help me create the graph. Thanks. denaturation of enzyme leading toloss of its activity and function. So,TDET will not be able to catalyse theconversion due to denaturationTDET requires GWRM for catalyzingthe conversion of the substanceTDET. WARN will bind to coenzymeGWRM which then cannot bind toenzyme TDET. Thus, TDET will not beable to catalyse the conversion ofSHARE to the product.You were asked to perform an experiment using spectrophotometric analysis. Thesystem that you are studying is a reaction catalyzed by the enzyme TDET and requiresthe coenzyme GWRM. In the reaction, substance SHARE is converted to a coloredsubstance CRMSN that can be detected spectrophotometrically. All other substanceswill not absorb at the Amax of CRMSN.T4 NoTest tube set-ups were prepared as follows:T1: SHARE + TDETT2: SHARE + TDET + GWRMT3: SHARE + TDET + GWRM + gradual heating until boilingT4: SHARE + TDET + GWRM + WARN*T5 NoTDET enzyme is required along withcofactor GWRM for convertingsubstance SHARE to the product. AsSHARE is absent, TDET enzymedoesn't have the substrate which itwill convert to the product.T5: TDET + GWRM*Substance WARN can bind to GWRM and prevent it from binding to TDET.Brief explanation for your answer.Do you expect productCRMSN to be formed overtime? [Yes/No]T1 NoBased on the given problem, create/draw a single graph, and plot the trend lines thatdepict the rate of CRMSN production in the five tubes over time.Because TDET enzyme requirescofactor GWRM for convertingsubstance TDET to the product andGWRM is absent. Thus, TDET will notbe able to catalyse the conversion.Because TDET enzyme requirescofactor GWRM for convertingsubstance SHARE to the product andGWRM is present.Thus, TDET will be able to catalysethe conversion to CRMSN.Do not forget to:Label the X and Y axes.T2 YesUse appropriate legends or label the trend lines.• Explain the generated graph.T3 NoBecause TDET enzyme will getdenatured at such high temperatureas high temperature causes

Enzymes are protein biomolecules which increase the rate of chemical reaction by decreasing the…

Answered: Based on the given problem, create/draw…

Based on the given problem, create/draw a single graph, and plot the trend lines that depict the rate of CRMSN production in the five tubes over time.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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