B. Pancreatin Digestion TUBE SIZE of egg white Results Purple colored Solution 1(Pancreatin) No change in size No change in size Purple colored Solution 2(Water) No change in size Light-Purple colored Solution 3(water, Pancreatin) |(Ctrl) Size decreased Blue colored solution 4(water, Pancreatin, Na2CO3) greatly

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Please help me explain the results on the table why is it like that.

Explain one by one per test tube

 topic is about protein digestion of pancreatic digestion

thank yyou

B. Pancreatin Digestion
TUBE
SIZE of egg white
Results
Purple colored Solution
1(Pancreatin)
No change in size
No change in size
Purple colored Solution
2(Water)
No change in size
Light-Purple colored Solution
3(water, Pancreatin)
|(Ctrl)
Size decreased
Blue colored solution
4(water, Pancreatin,
Na2CO3)
greatly
Transcribed Image Text:B. Pancreatin Digestion TUBE SIZE of egg white Results Purple colored Solution 1(Pancreatin) No change in size No change in size Purple colored Solution 2(Water) No change in size Light-Purple colored Solution 3(water, Pancreatin) |(Ctrl) Size decreased Blue colored solution 4(water, Pancreatin, Na2CO3) greatly
B. Pancreatin digestion
1. Prepare 4 test tubes as follows:
Tube 1 - 3 ml neutral pancreatin
Tube 2 - 3 ml water
Tube 3 - 3 ml water + 1.5 ml pancreatin
Tube 4 - 3 ml water + 1.5 ml pancreatin +1.5 ml 1% N22CO3
2. Place equal size of hard-boiled egg white in all tubes.
3. Place all tubes in water bath with a temperature maintained at 40°C for 1.5
hours.
4. Add 3 drops of toluene in all tubes and incubate till the next lab period.
5. Observe the size of the egg white in each of the tubes.
6. Take 1 ml of the supernatant in each tube and place in 4 separate test tubes
labelled 1b,2b,3b,4b. Add 1 ml of 10% NaOH and 2 drops of 1% CuSO4 in each
tube. Mix and compare the result of the 4 tubes.
Transcribed Image Text:B. Pancreatin digestion 1. Prepare 4 test tubes as follows: Tube 1 - 3 ml neutral pancreatin Tube 2 - 3 ml water Tube 3 - 3 ml water + 1.5 ml pancreatin Tube 4 - 3 ml water + 1.5 ml pancreatin +1.5 ml 1% N22CO3 2. Place equal size of hard-boiled egg white in all tubes. 3. Place all tubes in water bath with a temperature maintained at 40°C for 1.5 hours. 4. Add 3 drops of toluene in all tubes and incubate till the next lab period. 5. Observe the size of the egg white in each of the tubes. 6. Take 1 ml of the supernatant in each tube and place in 4 separate test tubes labelled 1b,2b,3b,4b. Add 1 ml of 10% NaOH and 2 drops of 1% CuSO4 in each tube. Mix and compare the result of the 4 tubes.
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