Assume the DpnI digestion has failed after the PCR in the synthesis of mutated plasmid by thermal cycling process. Under such circumstance, a) What kind of transformants would be found on the plate? Provide reason(s) for your answer. b) How would it affect the number of colonies that contain the mutant construct? Provide reason(s) for your answer. asap
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Assume the DpnI digestion has failed after the PCR in the synthesis of mutated plasmid by thermal cycling process. Under such circumstance,
a) What kind of transformants would be found on the plate? Provide reason(s) for your answer.
b) How would it affect the number of colonies that contain the mutant construct? Provide reason(s) for your answer.
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