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An ion exchange column is packed with 2.76 kg of DEAE (diethylaminoethyl) cellulose beads. On average, each bead weighs 0.0015 g and has an average of 2.88 * 10 12 positively charged amine groups that can adsorb a negatively charged protein that passes through the column. A solution containing 6.45 mg/L of a protein is maintained at a constant pH as it passes through the ion exchange column at 0.438 L/min. The protein has a molecular weight of 62,000. The pk of the amino groups on DEAE cellulose is 7.1, and the pl of the protein is 4.8. A. What range of pH for the feed solution will allow operation of the ion exchange column so that the protein will stick to the beads and remain in the column? B. Discuss what would happen if the pH of the protein solution is (i) too high or (ii) too low. C. Using a pH buffer in the range you listed for part A, how long can the column be operated before reaching 60% capacity (i.e., 60% of the amino groups on DEAE are bound to the protein through an ionic bond)? You may assume that one protein attaches to one + charge on the beads (although it's possible that proteins attach to more than one + charge). Assume that 100% of the proteins adhere to DEAE beads and no protein exits the column. D. After reaching 60% capacity, explain what you would do to release the protein attached to the beads. (to get the isolated product and regenerate the ionic exchange resin).

An ion exchange column is packed with 2.76 kg of DEAE (diethylaminoethyl) cellulose beads. On average, each bead weighs 0.0015 g and has an average of 2.88 * 10 12 positively charged amine groups that can adsorb a negatively charged protein that passes through the column. A solution containing 6.45 mg/L of a protein is maintained at a constant pH as it passes through the ion exchange column at 0.438 L/min. The protein has a molecular weight of 62,000. The pk of the amino groups on DEAE cellulose is 7.1, and the pl of the protein is 4.8. A. What range of pH for the feed solution will allow operation of the ion exchange column so that the protein will stick to the beads and remain in the column? B. Discuss what would happen if the pH of the protein solution is (i) too high or (ii) too low. C. Using a pH buffer in the range you listed for part A, how long can the column be operated before reaching 60% capacity (i.e., 60% of the amino groups on DEAE are bound to the protein through an ionic bond)? You may assume that one protein attaches to one + charge on the beads (although it's possible that proteins attach to more than one + charge). Assume that 100% of the proteins adhere to DEAE beads and no protein exits the column. D. After reaching 60% capacity, explain what you would do to release the protein attached to the beads. (to get the isolated product and regenerate the ionic exchange resin).

Introduction to Chemical Engineering Thermodynamics
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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An ion exchange column is packed with 2.76 kg of DEAE (diethylaminoethyl) cellulose beads. On average, each bead weighs 0.0015 g and has an average of 2.88 * 10 12 positively charged amine groups that can adsorb a negatively charged protein that passes through the column. A solution containing 6.45 mg/L of a protein is maintained at a constant pH as it passes through the ion exchange column at 0.438 L/min. The protein has a molecular weight of 62,000. The pk of the amino groups on DEAE cellulose is 7.1, and the pl of the protein is 4.8. A. What range of pH for the feed solution will allow operation of the ion exchange column so that the protein will stick to the beads and remain in the column? B. Discuss what would happen if the pH of the protein solution is (i) too high or (ii) too low. C. Using a pH buffer in the range you listed for part A, how long can the column be operated before reaching 60% capacity (i.e., 60% of the amino groups on DEAE are bound to the protein through an ionic bond)? You may assume that one protein attaches to one + charge on the beads (although it's possible that proteins attach to more than one + charge). Assume that 100% of the proteins adhere to DEAE beads and no protein exits the column. D. After reaching 60% capacity, explain what you would do to release the protein attached to the beads. (to get the isolated product and regenerate the ionic exchange resin).
Transcribed Image Text:An ion exchange column is packed with 2.76 kg of DEAE (diethylaminoethyl) cellulose beads. On average, each bead weighs 0.0015 g and has an average of 2.88 * 10 12 positively charged amine groups that can adsorb a negatively charged protein that passes through the column. A solution containing 6.45 mg/L of a protein is maintained at a constant pH as it passes through the ion exchange column at 0.438 L/min. The protein has a molecular weight of 62,000. The pk of the amino groups on DEAE cellulose is 7.1, and the pl of the protein is 4.8. A. What range of pH for the feed solution will allow operation of the ion exchange column so that the protein will stick to the beads and remain in the column? B. Discuss what would happen if the pH of the protein solution is (i) too high or (ii) too low. C. Using a pH buffer in the range you listed for part A, how long can the column be operated before reaching 60% capacity (i.e., 60% of the amino groups on DEAE are bound to the protein through an ionic bond)? You may assume that one protein attaches to one + charge on the beads (although it's possible that proteins attach to more than one + charge). Assume that 100% of the proteins adhere to DEAE beads and no protein exits the column. D. After reaching 60% capacity, explain what you would do to release the protein attached to the beads. (to get the isolated product and regenerate the ionic exchange resin).
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