An enzyme reaction takes place in a 10mL solution that has a total citrate concentration of 120mM and an initial pH of 7.00. During the reaction, 0.2mmoles of acid are produced. (pKa values for citric acid: pKa1=3.15, pKa2=4.77, pKa3=6.40) a) At pH 7.00 (before the enzyme reaction occurs), determine the concentrations of the predominant forms of citrate in solution. b) calculate the pH of the solution after the enzymatic reaction has occurred.

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Chapter20: Enzymes
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Problem 20.22E
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An enzyme reaction takes place in a 10mL solution that has a total citrate concentration of 120mM and an initial pH of 7.00. During the reaction, 0.2mmoles of acid are produced. (pKa values for citric acid: pKa1=3.15, pKa2=4.77, pKa3=6.40)

a) At pH 7.00 (before the enzyme reaction occurs), determine the concentrations of the predominant forms of citrate in solution.

b) calculate the pH of the solution after the enzymatic reaction has occurred. 

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