### Exam Questions on Amino Acids and Isoelectric Focusing#### 29. Which amino acid is ideal for the transfer of protons within the catalytic site of enzymes due to the presence of significant amount of both the protonated and deprotonated forms of its side chain at biological pH? A. **Lysine**B. **Asparagine**C. **Tyrosine**D. **Cysteine**E. **Histidine**#### 30. The isoelectric point of alanine is pH = 6.15. It is mixed with proline (pKa1 = 2.0; pKa2 = 10.6). The mixture is placed in an electric field at pH 6.15 and then subjected to isoelectric focusing. Which statement is true?A. **The two amino acids will be separated**B. **The two amino acids will not be separated**C. **Neither amino acids will move in the electric field**D. **Both amino acids will move from the origin and separated**---**Detailed Explanation of Relevant Concepts:**1. **Ideal Amino Acid for Proton Transfer at Biological pH:** - Histidine is commonly known for its ability to shuttle protons during enzymatic reactions due to the pKa of its side chain (approximately 6.0), making it well-suited for proton transfer at physiological pH (~7.4).2. **Isoelectric Focusing:** - Isoelectric focusing separates amino acids based on their isoelectric points (pI). - Alanine, with a pI at 6.15, will be neutral at pH 6.15 and thus will not move within the electric field. - Proline, depending on its net charge at pH 6.15 (considering its pKa values), will have either a positive or negative charge and thus will migrate until it reaches a position in the gradient where the pH matches its isoelectric point.This information provides essential background knowledge to answer the questions accurately for educational purposes.

Histidine is an amino acid that possesses a unique property in its side chain. At biological pH,…

29.Which amino acid is ideal for the transfer of protons within the catalytic site of enzymes due to the presence of significant amount of both the protonated and deprotonated forms of its side chain at biological pH? A. Lysine B. Asparagine C. Tyrosine D. Cysteine E. Histidine = 2.0; pka2 30. The isoelectric point of alanine is pH = 6.15. It is mixed with proline (pka1 10.6). The mixture is placed in an electric field at pH 6.15 and then subjected to isoelectric focusing. Which statement is true? A. The two amino acids will be separated B. The two amino acids will not be separated C. Neither amino acids will move in the electric field D. Both amino acids will move from the origin and separated

29.Which amino acid is ideal for the transfer of protons within the catalytic site of enzymes due to the presence of significant amount of both the protonated and deprotonated forms of its side chain at biological pH? A. Lysine B. Asparagine C. Tyrosine D. Cysteine E. Histidine = 2.0; pka2 30. The isoelectric point of alanine is pH = 6.15. It is mixed with proline (pka1 10.6). The mixture is placed in an electric field at pH 6.15 and then subjected to isoelectric focusing. Which statement is true? A. The two amino acids will be separated B. The two amino acids will not be separated C. Neither amino acids will move in the electric field D. Both amino acids will move from the origin and separated

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

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