An analyst was told to determine the amount of glucose in blood using a colorimetric method. The analyst prepared a series of standards containing known amounts of glucose and obtained the results given in the table below by UV/visible spectroscopy. Concentration of Glucose/mmol L-¹ 0.0 2.0 4.0 6.0 8.0 10.0 12.0 Absorbance 0.11 0.25 0.45 0.63 0.87 1.05 1.24 Use the data in the table to plot a graph of absorbance against glucose concentration. a) Is Beer's Law obeyed? b) From the best fit line, calculate the molar absorptivity, &, and its units. c) If a urine sample has absorbance = 0.53, use your graph (not any individual data points) to determine its glucose concentration.

An analyst was told to determine the amount of glucose in blood using a colorimetric method. The analyst prepared a series of standards containing known amounts of glucose and obtained the results given in the table below by UV/visible spectroscopy. Concentration of Glucose/mmol L-¹ 0.0 2.0 4.0 6.0 8.0 10.0 12.0 Absorbance 0.11 0.25 0.45 0.63 0.87 1.05 1.24 Use the data in the table to plot a graph of absorbance against glucose concentration. a) Is Beer's Law obeyed? b) From the best fit line, calculate the molar absorptivity, &, and its units. c) If a urine sample has absorbance = 0.53, use your graph (not any individual data points) to determine its glucose concentration.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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