Alkaline phosphatase catalyzes the hydrolysis of various phosphate monoesters, including p-nitrophenyl phosphate (RNPP). Inorganic phosphate (P.) and phenylalanine (Phe) are inhibitorsfor alkaline phosphatase. The following results were obtained for alkaline phosphatase-catalyzedDNPR hydrolysis at fixed initial enzyme concentration, in the absence and presence of P, andPhe:IRNPPI (mM)Vo (M/min)Vo (M/min)with 1 mM P0.0143Vo (M/min)with 10 mM Phe.0.5460.02140.01020.2670.01750.01090.009350.1630.01340.006670.007830.1070.01070.005380.006750.0820.008860.004100.006140.0540.006500.00290(a) Draw the structures of the substrate (pNPP) and of the inhibitors (P, and Phe). Considerthem in the fully deprotonated states. What similarities can you detect between eachinhibitor and the substrate?

Para-Nitrophenylphosphate is a non-proteinaceous chromogenic substrate for alkaline and acid…

Alkaline phosphatase catalyzes the hydrolysis of various phosphate monoesters, including p- nitrophenyl phosphate (RNPP). Inorganic phosphate (P.) and phenylalanine (Phe) are inhibitors for alkaline phosphatase. The following results were obtained for alkaline phosphatase-catalyzed RNPR hydrolysis at fixed initial enzyme concentration, in the absence and presence of P, and Phe: IENPPI (mM) Vo (M/min) Vo (M/min) with 1 mM P Vo (M/min) with 10 mM Phe. 0.0102 0.546 0.0214 0.0143 0.267 0.0175 0.0109 0.00935 0.163 0.0134 0.00667 0.00783 0.107 0.0107 0.00538 0.00675 0.082 0.00886 0.00410 0.00614 0.054 0.00650 0.00290 (a) Draw the structures of the substrate (PNPP) and of the inhibitors (P, and Phe). Consider them in the fully deprotonated states. What similarities can you detect between each inhibitor and the substrate?

Alkaline phosphatase catalyzes the hydrolysis of various phosphate monoesters, including p- nitrophenyl phosphate (RNPP). Inorganic phosphate (P.) and phenylalanine (Phe) are inhibitors for alkaline phosphatase. The following results were obtained for alkaline phosphatase-catalyzed RNPR hydrolysis at fixed initial enzyme concentration, in the absence and presence of P, and Phe: IENPPI (mM) Vo (M/min) Vo (M/min) with 1 mM P Vo (M/min) with 10 mM Phe. 0.0102 0.546 0.0214 0.0143 0.267 0.0175 0.0109 0.00935 0.163 0.0134 0.00667 0.00783 0.107 0.0107 0.00538 0.00675 0.082 0.00886 0.00410 0.00614 0.054 0.00650 0.00290 (a) Draw the structures of the substrate (PNPP) and of the inhibitors (P, and Phe). Consider them in the fully deprotonated states. What similarities can you detect between each inhibitor and the substrate?

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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