After Heat the mixture to separate mRNA and cDNA strands, and then eliminate the mRNA transcript. The 3’ ends of the cDNA strands loop around and bind by chance to complementary nucleotides within the same strand, forming a “hairpin loop” that serves as the primer for DNA polymerization, what is step 4 of making a cDNA library?
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
After Heat the mixture to separate mRNA and cDNA strands, and then eliminate the mRNA transcript. The 3’ ends of the cDNA strands loop around and bind by chance to complementary
cDNA (complementary deoxyribonucleic acid) is a synthetic nucleic acid molecule synthesized in-vitro with the use of mRNA (messenger ribonucleic acid) as a template. The reverse transcriptase (RT) enzyme is a specialized enzyme that performs this function. The cDNA molecule is devoid of any non-coding regions like regulatory sequences and introns.
Step by step
Solved in 2 steps
